Assist in the development of methods for related to the testing and characterization of biotherapeutics using mass spectrometry. Under minimal supervision, may be responsible for method development, client interaction, interpretation and reporting of data and regulatory compliance of assigned research projects.
ESSENTIAL DUTIES AND RESPONSIBILITIES:
•Perform and troubleshoot laboratory experiments, test, and procedures within accepted regulatory compliance for the characterization and analysis of biotherapeutics by mass spectrometry
•Perform routine testing techniques such as the following with a high degree of reliability, efficiency and accuracy:
oPeptide Mapping LC-UV/MS (MS)
oIntact Molecular Weight Analyses LC-UV/MS
oSample Preparation such as SDS PAGE, SPE, HPLC Peak Collection
oGlycosylation Mapping via Peptide Mapping LC-MS/MS
oN- and O- Linked Glycosylation Profiling
oDe novo Amino Acid Sequencing
oVarious methodologies developed for QTOF, Triple Quadrupole, and MALDI-TOF
oUse of Agilent and Thermo MS Platforms
•Review, interpret, integrate and present experimental data using assistance of senior technical staff as appropriate.
•Assist in reviewing procedures and data.
•Generate high-quality protocols and reports in conjunction with senior research personnel.
•Provide technical guidance to junior staff.
•Assist in long-term projects (redesign of SOPs, STMs, basic research procedures or report formats).
•Interact with the client regarding project design, scheduling and conduct as requested.
•Collaborate with appropriate departments to coordinate performance of projects, discuss issues and prepare reports.
•Attend scientific meetings, conferences and training courses to enhance job and professional skills.
•Perform other related duties as assigned.Qualifications
•Education: Bachelor’s degree (B.A. /B.S.) or equivalent in Chemistry/Biotechnology or related scientific discipline. M.S. or Ph.D. preferred.
•Experience: Minimum of three years’ experience within a contract research, academic or pharmaceutical industry, including experience in the core competencies of characterization and analysis of biotherapeutics.
•An equivalent combination of education and experience with contract research or the pharmaceutical industry may be accepted as a satisfactory substitute for the specific education and experience listed above.
•Other: Ability to work on problems of moderate scope where analysis of situation or data requires a review of identifiable factors. Exercises judgment within defined procedures and practices to determine appropriate action. Effective written and verbal communication skills with the ability to handle multiple projects, prioritize work and meet deadlines within a cGMP environment. Capable of data interpretation of basic characterization and analysis of biotherapeutics (glycoproteins and peptides) using mass spectrometry. Capable of anticipating and recognizing potential problems. Must be able to follow written and verbal instructions and possess good interpersonal skills that are conducive to effective communication.
•While performing the duties of this job, the employee is regularly required to effectively communicate regarding study conduct with internal personnel and external scientific associates and client.
•Regularly observes, inspects and interprets laboratory experiments, requiring close vision and the ability to adjust focus and detect and differentiate colors.
•Must be able to wear a respirator in compliance with OSHA regulations.
•General office and laboratory working conditions, the noise level in the work environment is usually quiet.
•While performing the duties of this job the employee is regularly required to wear personal protective equipment (safety glasses or goggles, face shield, gloves, respirator et al.), work near toxic or caustic chemicals, and work with biohazards.
•The employee may occasionally be exposed to fumes or airborne particles, unpleasant odors, and extreme temperatures.
Equal Employment Opportunity
Charles River Laboratories, Inc. is an Equal Opportunity Employer M/F/Disabled/Vet