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Summary

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Experiences

Current Experience

  • Stability Lab Assistant Strombeek, Grimbergen, Belgium
    Since June 2016

    -Stability follow-up of Hand Dish products (Dreft, Dawn, Fairy, Joy, etc.). 

    • pH, viscosity and colour measurements
    • Visual assessement 

    -Entering the results on Electronic Lab notebook (ELN) and on the data base (GAIMS).

    -Stability rooms management.

     

     

Past Experience

  • Assistant-PhD student Anderlecht, Belgique
    October 2014 --- November 2015

    Cultures, treatments, transfections (plasmids) of CHO TTA cells.
    Analysis of proteins expression by Western Blots, immunohistochemistry and immunofluorescence.
    Fluorescence microscopy (Axiovert 200M)
    Bradford protein assay.
    Sectioning (Microtome), fixing, stainings of tissues.
    Cervical translocation and dissections of mices.
    Plasmids sequencing.
    Researching and processing of scientific papers.
    Teaching of practical lessons in histology (medicine faculty).

    Bacteria culture (miniprep, maxiprep, transformation)

  • Intern Anderlecht, Belgique
    September 2012 --- December 2013

    1. FRIA project: Effects of PACAP and VIP on inflammation and triglycerides metabolism in adipocytes.
      Researching and processing of scientific papers.
      Writing and presentation of a PhD project in a view to obtain a grant (FRIA).
    2. Master’s thesis: Regulation of fatty acids receptors expression on adipocytes.
      (Promotor: Professor Christine Delporte)
      Cultures, differentiations and treatments of 3T3-L1 cells.
      Red oil stainings to check the cells differentiation.
      Analysis of proteins expression by Western Blots.
      Analysis of mRNA expression by q-RT-PCR.
      mRNA extraction, cDNA synthesis and verification of RNAs quality.
      RNA dosing by nanadrop.
      Bradford protein assay.
      Statistical analysis of the results (SPSS).
      Researching and processing of scientific papers.
      Writing and presentation of Master’s thesis.

  • Intern Anderlecht, Belgique
    January 2013 --- January 2013

    Internship: MicroRNA functional study on thyroid tumors.

    Cultures, transfections (microRNAs) of HEK cells.
    Analysis of HEK cells migration by scratch assay.
    Validation of mRNAs regulation by microRNAs via q-RT-PCR.
    Learning and practice of the following techniques: luciferase assay, site-directed mutagenesis (PCR, gel electrophoresis, bacteria transformation, miniprep, restriction and sequencing).
    Researching and processing of scientific papers.
    Writing of internship report.

  • Intern Anderlecht, Belgique
    March 2012 --- March 2012

    Internship: Effects of VIP and PACAP on adipocyte differentiation.

    Cultures, differentiations and treatments of 3T3-L1 cells.
    Red oil stainings to check the cells differentiation.
    Analysis of proteins expression by Western Blots.
    Analysis of mRNA expression by q-RT-PCR.
    mRNA extraction, cDNA synthesis and verification of RNAs quality.
    RNA dosing by nanadrop.
    Bradford protein assay.
    Statistical analysis of the results.
    Researching and processing of scientific papers.
    Writing of internship report.

  • Intern Anderlecht, Belgique
    December 2011 --- December 2011

    Internship: Mechanisms involved in beta cells apoptosis caused by fatty acids-induced ER stress in type 2 diabetes.

    Cultures and treatments of ß-cells and INS-E.
    Viability evaluation of the cells.
    Learning and practice of the following techniques: western blots and RNA extraction.
    Statistical analysis of the results (SPSS).
    Researching and processing of scientific papers.
    Writing of internship report.

Personality

BrightOwl Assessment :
ResiliencyAnger commandIndependencePerspectiveCuriosityDiligenceOrganizationDependabilityAttention to detailEfficiencySelf-discipline
Self Assessment :
IndependenceCollaborationSociabilityProactivityKindnessCreative thinkingAttention to detailAdaptabilityInterest in knowledgeFlexibilityEfficiencyProblem solvingStrivingCuriositySelf-disciplineOrganization

Knowledge

BrightOwl Assessment :
In VitroIn VivoData AnalysisCell CulturePCRWestern BlottingStatistical Data AnalysisImmunofluorescence
Self Assessment :
DiabetesMicroscopyWestern BlottingNeuroscienceProtein ExpressionTransfectionSDS-PAGETime ManagementRT-PCRStatisticsR&DQuantitative PCR (qPCR)Scientific methodologymolecular biologyMicrosoft OfficeHematologyMicrobiologyDNA sequencingImmunohistochemistry (IHC) Cell biology Biochemistry Animal models R&DAntibodiesCell CultureBiochemistryDNA extractionDNAEnzyme-linked immunosorbent assay (ELISA)EnglishFluorescent microscopyImmunofluorescenceIn VitroIn VivoPathology

Skills and Expertise

BrightOwl Assessment :
Written presentation Analyze dataInterpret dataManage data reporting systemsManage data collection system Report data Guide studentsExtrapolate data
Self Assessment :
Develop protocolsDesign studyInterpret dataOral presentationExecute scientific projectsPrepare final reportStatistical analysisUse western blotting techniqueWritten presentationData analysisAnalyze proteinAlzheimerlifescience Guide students Interpret dataAttend seminarsCommunicate with investigatorResearch at universities

Education

  • University graduate in Sciences and techniques for laboratory animals from ULB in 2014
  • Master in Biomedical Sciences from ULB in 2013
  • Bachelor in Biomedical Sciences from ULB in 2011

Training and Certification

  • Sciences and techniques of laboratory animals in 2014 Certification

Languages

BrightOwl Assessment:
French
Native
Dutch
Elementary Proficiency
English
Elementary Proficiency
Self Assessment:
French
Native
Dutch
Professional Proficiency
English
Professional Proficiency

Work Preferences

  • Positions I am interested in:
    Clinical Data Manager (DM) Clinical Trial Assistant (CTA) Clinical Research Associate (CRA)
  • Locations I am interested in:
    Brussels, Belgium East Flanders, Belgium Flemish Brabant, Belgium Hainaut, Belgium Mechelen, Belgium Namur, Belgium Waals-Brabant, Belgium
  • Work From Home:
    Yes, 0 to 1 days per week
  • Work Regime:
    Permanent position
    BrightOwl employee
  • International:
    Yes

Area / Region

Brussels, Belgique

Others

Driving License
  • Yes