Senior Scientist Biology and in vitro Pharmacology Diepenbeek, Belgium
July 2011 --- November 2015
Responsible for setup, validation and management of biochemical and cellular in vitro assays required for in vitro pharmacology of kinase inhibitors to unravel the mechanism of action new therapeutics focused on lung, eye and bowel diseases. Responsible for internal in vitro cell culture studies and stability assays - Leading screening of Amakem compounds in internal available cellular based assays - Literature search for new techniques – targets – assays - Setting-up new cellular assays for screening of compounds (new primary cells, new analysis methods) and validation of new assays - Setting-up new analysis techniques for e.g. PK samples - Analysis of all internal obtained data, reporting and presenting to VP R&D Managing the daily activities of 3 technicians and associate scientists. Follow-up external in vitro/stability studies with academic collaborators (KULeuven, IBD-UGent Kyoto JP, University of Aachen, DE)
Postdoctoral Researcher Liège, Belgium
September 2007 --- June 2011
Identification and evaluation of inhibitors of angiogenesis suitable for the treatment of angiogenesis and/or lymphangiogenesis- related diseases such as cancer. Biochemical and molecular biological techniques, several in vitro and in vivo cancer models including in vivo imaging (intravital microscopy, bioluminescent in vivo imaging.
Fellowship Turku, Finland
August 2007 --- September 2007
The role of vascular adhesion protein 1 (VAP-1) in the recruitment of myeloid cells into the tumor and its effect on tumor angiogenesis. Molecular biological techniques (ELISA, qRT-PCR), cell culture (flow cytometry), immunohistochemistry, mouse tumor models, intravital microscopy.
PhD student Maastricht, The Netherlands
September 2003 --- September 2007
Investigated the relationship between angiogenesis and the immune system in cancer. Cell biological techniques (cell culture, flow cytometry, cell isolation from tissues, leukocyte adhesion assays), in vivo tumor growth models, intravital microscopy, immunohistochemistry, molecular cell biological techniques such as RNA-DNA isolations from cells and tissues, real time qRT-PCR and Western blots.
Technician Maastricht, Netherlands
September 2002 --- August 2003
Project: Improving immunotherapy by using inhibitors of angiogenesis. Investigate the effects of different angiostatic treatments and immunotherapy in mouse tumor models and their effect on leukocyte tumor infiltration. Techniques included cell culture, flow cytometry in vivo studies and immunohistochemistry.
November 2001 --- August 2002
Quality control of different raw materials in the nutrition industry
AdaptabilityAnalytical thinkingAttention to detailCollaborationCoordinationCreative thinkingCritical thinkingCuriosityEfficiencyFlexibilityInterest in knowledgeOrganizationProblem solvingSociability
Skills and Expertise
Create SOPs Analyze data Control data Develop protocols Guide students Interpret data Receptor Pharmacology Report data Search literature on clinical trials Write papersAdjust processes and methodsAdjust processes Adverse event reportingAttend seminarsBiomarker ResearchCollaborate with project teamCollect dataConduct research at universitiescoordinating research projectsCreate SOPsData validationDesign scientific projects (in biology)Direct co-workersExecute scientific projectsGastrointestinal diseasesInteract with CROsInteract with pre-clinical scientistslifescienceManage laboratory proceduresmanaging a small teamManuscript preparation and reviewOral presentationPrepare final reportPresent data at congressProtocol managementPublication of articlesReview data interpretationSetup of research equipmentSupervise PhD studentsSupervise technicians
Master’s Degree in Biology (major physiology-biochemistry) from University of Antwerp in 0