Scientist in a custom oligonucleotides company and academic background in immunology and neurobiology. Involved in drug discovery projects and in studies on the molecular mechanisms of learning and memory. Since 2015, employee in a DNA synthesis lab. Design and development of real-time PCR assays and customer support. Working Expertise and methodological know-how in fundamental and applied research, in areas such as genetics, molecular and cell biology, biochemistry and animal physiology. Responsibility as an independent project leader by steering and controlling several projects and being an active team member. High performance, professional working style dealing with daily demands in a flexible way. Supervision of teams of up to 6 members. Involved in laboratory organisation, teaching and communication. Working in an ISO 9001 ISO 13485 company. Taking up new challenges to enrich my knowledge in life sciences but also in the understanding of human beings and modern society.
Scientist - Designer and customer support Berlin, Germany
Since April 2015
Design and development of real-time PCR assay (TaqMan probes, SimpleProbe, Hybridisation probes, molecular beacons) for quantification, genotyping (mutation analysis) and discrimination, in single detection and multiplex formats. Support for other DNA-based application (in situ hybridisation, methylation assays). Customer support: Assay design, information on our portfolio (research use only and CE in vitro diagnostic kits), sales and support by troubleshooting. Participation to the certification of CE IVD kits (Technical documentation, translation work). TIB Molbiol is an ISO 9001 /ISO 13485 company.
March 2015 --- March 2015
Design of real-time PCR assay (TaqMan probes, SimpleProbe)
Principal investigator - Wissenschaftliche Mitarbeiter
August 2012 --- August 2014
DFG (Deutsche Forschungsgemeinschaft) Research Grant: “Molecular correlates of memory phases and development of molecular tools to modify brain physiology in the honeybee”. Applicant: Dr. Gérard Leboulle. Budget: 210,000 €. I analysed the molecular mechanisms of learning and memory in the honeybee Apis mellifera and performed neuroanatomical studies. I focused on the glutamate neurotransmission and glutamate chloride channels. Active in project management (planning of budget experiments, collaboration and work at the bench) and laboratory organisation. Documentation and communication of results.
Managing of ongoing projects
May 2011 --- August 2012
Principal Investigator - Wissenschaftliche Mitabreiter Berlin, Germany
March 2008 --- April 2011
BMBF (Bundes Ministerium für Bildung und Forschung) FUGATO-joint programme. Title: “Entwicklung molekulargenetischer Methoden für die Selektion krankheitsresistenter Honigbienen basierend auf Genexpressionsunterschieden von hygienischen und nicht hygienischen Arbeitsbienen”. I participated to the writing of the project. Budget: 820,250 €. I was involved in a joint project on hygienic behaviour that might be economically relevant. Studies on the glutamatergic neurotransmission and on the development of molecular tools to modulate brain physiology were continued. Project management (planning of budget and experiments, work at the bench) and laboratory organisation. Leadership of a research group of up to 4 members. Documentation and communication of results and involvement in teaching and in several collaborations.
Managing of ongoing projects
July 2007 --- February 2008
Principal investigator - Wissenschatliche Mitarbeiter Berlin, Germany
January 2004 --- June 2007
DFG (Deutsche Forschungsgemeinschaft) Research Grant: “A molecular approach to describe the role of glutamate NMDA receptor in memory formation and behaviour of an insect model: the honeybee Apis mellifera”. Applicant: Dr. Gérard Leboulle. Budget: 210,000 €. DAAD (Deutscher Akademischer Austauschdienst) grant: “The Role of Glutamate Receptors in Learning and Memory in the brain of Apis mellifera”. Applicant: Dr. Gérard Leboulle. Budget: 5,850 €. I initiated a study on the glutamatergic neurotransmission of the honeybee Apis mellifera, by focusing on NMDA glutamate receptor and glutamate chloride channel. As a member of the “Honeybee Genome Consortium”, I contributed to the annotation process of the genome. I worked on the development of molecular tools to modify specifically gene expression in the brain. Project management (planning of budget and experiments, work at the bench) and laboratory organisation. Leadership of a research group of up to 6 members, documentation and communication of results and involvement in teaching and in several collaborations.
Postdoc - Invited Scientist Berlin, Germany
October 2002 --- December 2003
I studied the modality of cAMP dependent protein kinase (PKA) activation. The development of molecular tools to modulate brain physiology was undertaken.
Analytical thinkingAttention to detailCommunicativeCoordinationCreative thinkingCritical thinkingCuriosityEfficiencyFlexibilityInnovative thinkingInterest in knowledgeKindnessOptimismOrganizationOrientationProblem solvingReaction to stressResponsibilityResult OrientedService orientedSociabilityWillingness to compromiseTrust
Animal models Biostatistics Biochemistry R&D Scientific writingAccount ManagementAnalysisApplicationsAseptic TechniquesAssay developmentAutoclaveBioanalysisBiochemistryBioinformaticsBiological Drug DevelopmentBiologyBiomarkersBiotechnologyBudgetsCE CertificationCell CultureCell Based AssaysCell SignalingCharacterizationChromatographyCNSCommunication SkillsComputational BiologyConfocal MicroscopyContinuous ImprovementCustomer ServiceData AnalysisDatabasesDiagnosticsDNADrug DiscoveryEarly development stageElectrophoresisELISAEndocrinologyEnglishEnzyme AssaysEnzyme-linked immunosorbent assay (ELISA)Event ManagementExperimentationGel ElectrophoresisGenetic polymorphismGeneticsGenomicsGermanGood Laboratory Practice (GLP)grant writing and designHistologyImmunoassaysImmunofluorescenceImmunohistochemistry (IHC)ImmunologyIn VitroIn vitro diagnostics (IVD)In VivoInfectious diseasesInflammationISOISO 13485ISO 9001It skillsLaboratory ManagementLaboratory ResearchLaboratory SkillsLeadershipLife SciencesMammalian Cell CulturemanagementMatlabMicrobiologyMicroscopyMolecular & Cellular BiologyMolecular DiagnosticsMultilingualNeurosciencePatentsPCRpeople managementPhysiologyPresentation SkillsProblem SolvingProduct developmentProject ManagementProtein ExpressionProtein PurificationqPCRrecombinant DNA technologyResearchRNA isolationSalesSequence AnalysisSequencingStandard Operating Procedure (SOP)StatisticsSterilizationSystems BiologyTeachingTechnical SupportTransfectionTranslationTroubleshootingWestern Blotting
Skills and Expertise
Analytical skills Analyze data Create SOPs Develop protocols Guide students Interpret data Molecular Diagnostics Use a confocal microscopy Write papers Write protocolsAdjust processes and methodsAnalyze proteinAssess product quality issuesAssist with experimentsAttend seminarsAttend seminars, courses and meetings within and outside the companyBiomarker ResearchCell cultureCoach staffcollaborate in research projects at universitiesCollaborate with project teamCollect dataCommunicate with investigatorCommunicationConduct animal studiesConduct limited data and/or statistical analysis.Conduct literature searchesConduct research at universitiesConduct studiesCoordinate projectsCreate SOPsCreates a collaborative team environmentDesign protocolDesign scientific experimentsDesign scientific projects (in biology)Design studiesdrafting grant applicationsExecute scientific projectsFollow up projectsGuide staffGuide studentsLead teamsLead studieslifescienceManage budgetsManage research projectsmanaging a small teamManuscript preparationManuscript preparation and reviewManuscript reviewObtain grantsOral presentationOrganize scientific projects (in biology)pcrPlan work to meet objectives and deadlinesPrepare final reportPrepare manuscriptproject managementProvide technical supportProvide support for scientific and/or technical projectsPublish scientific projectsR&DResearch at universitiesReview dataSet up research projectsSolve problemsSpecimen processingStatistical analysisSupervise analysts and researchersSupervise PhD studentsSupervise techniciansTeach university studentsTeachingUse fluorescence microscopyUse laboratory techniquesUse western blotting techniqueWrite final reportsWrite documentsWritten presentation
PhD in molecular biology in Immunobiology and parasitology from Université libre de Bruxelles in 2002
Licence en Sciences Zoologiques (Master's degree) in Immunobiology from Université libre de Bruxelles in 1996
Secondary school in from Athénée Royal de Waterloo in 1992
Training and Certification
Saftey officer for medical products in 2015 Training
Technical Documentation in-vitro diagnostic in 2015 Training
Biosafety regulation in 2010 Training
Expert has 18 publications (Will be avalible with full profile)