Senior Research Scientist/Senior Cell Biologist
Since August 2015
Group focused on Reagent & assay development and disease modeling using human stem cells and iPSCs. • Generated several transgenic human iPS cell lines through precise genome edition using CRISPR/Cas9 & ObliGaRe to support drug discovery programs in neuroscience, cardiovascular & metabolic disease area (diabetes). • Lead & managed AstraZeneca collaboration with stemBANCC & EBiSC consortium. • Established efficient methods to differentiate iPSCs into CPCs, EPDCs & Cardiomyocytes.
June 2014 --- August 2015
Group focused on assay development and disease modeling using human stem cells and iPSCs. Senior Scientist • Established human stem cell and iPSCs platform to support various programmes including ALS, SMA & HD. • Developed multiple cell-based assays using stem cells, iPSCs and rodent primary cells. • Trained several scientists at biopolis and several other GSK R&D sites.
November 2010 --- June 2014
Lab focused on drug discovery and disease modeling using human ESCs & human iPSCs. Postdoctoral researcher • Research focused on developing novel therapeutics for ALS disease through high content small molecule screen using motor neurons derived from embryonic stem cells (ESCs). • Modeling ALS disease using human ESCs and human patient derived iPSCs. • Used molecular biology, cell biology, tissue culture, histology, flow cytometry, virology and imaging techniques extensively. Manages mouse colony. • Wrote postdoctoral fellowship, trained undergraduates student and technicians in various techniques. • Trained and supervised undergrads for dissertation project.
November 2009 --- October 2010
Lab focused on understanding zebrafish ciliogenesis. Postdoctoral fellow • Research focused on creating stable transgenic zebrafish lines for intra-flagellar transport (IFT) genes. • Used gene delivery, molecular biology, histology and imaging techniques very extensively. Manages zebrafish facility.
PhD in Neuroscience from National Brain Research Centre, Gurgaon in 2010
MSc in Biotechnology from Deendayal Upadhyay Gorakhpur University in 2002
BSc in Biology from St. Andrews College Gorakhpur in 1998