Senior Research Scientist
May 1990 --- January 2014
Development of classical subunit and recombinant veterinary vaccines against parasites e.g. recombinant Boophilus microplus vaccine, subunit Babesia canis vaccine (Nobivac®Piro), 2nd generation recombinant Babesia canis vaccine, subunit Babesia rossi vaccine, recombinant Babesia divergens vaccine. Development of in vitro culture systems, both stationary and small scale fermentor, for parasites e.g. Babesia canis, Babesia rossi or Babesia divergens to produce antigens. Design of large scale production systems for the cultivation of parasites and transfer to responsible production departments. Purification and standard biochemical analysis of both native and recombinant antigens, using e.g. IEF, SDS PAGE, preperative IEF, SDS PAGE, Western blotting, precipitation or chromatography techniques. Identification of novel vaccine candidates using mass spectrometry (MS/MS). Responsible for preparation and optimization of vaccine formulations, vaccine stability studies for registration purposes, vaccine component analysis. Setup of animal studies for efficacy or GLP safety, antigen titration studies. Data analysis of in vivo studies and reporting studies to management. Design of Quality Control (QC) assays (ELISA, Alpha-ELISA) for antigen production and product release or antibody titer determination. Transfer of assays to responsible QC departments. Development of cell biological assays eg inhibition assay, TLR reporter gene assays, LST assays, cytokine assays or in vitro feeding assay of tick larvae. Collaboration with research groups in France, South Africa and Mexico. Guidance and supervision of both local and foreign students.
Head laboratories Parasitology R&D
January 1995 --- January 2010
Leading a team of 10 people, responsible for coordination and implementation of Good Laboratory Practice (GLP) and Safety, Health & Enviroment (SHE) policies. Maintenance of laboratories buildings and equipment, Standard Operating Procedures (SOP). Responsible for internal and external GLP/SHE audits. Contact person for internal and external departments/firms. Buyer and validation (IQ/OQ/PQ) of new equipment. Unification of GLP policy for R&D/D&T in collaboration with QA department.
February 1988 --- May 1990
Division of Gastrointestinal and Liver Diseases. PhD research project: Identification of putative hepatocyte receptors for Plasmodium falciparum.
March 1987 --- February 1988
Research project: Cell lineage research of the mouse embryonic endoderm.
May 1984 --- March 1987
Division of Gastrointestinal and Liver Diseases PhD research projects: * Molecular regulation of Fibrinogen and Albumin synthesis during the Acute Phase Response. * Regulation of pancreatic polypeptides.
HLO (HBO-B) in Biochemical Technician from HMLS Oss in 1984
HLO (HBO-A) in Botanical Technician from STOVA Wageningen in 1980