Specialties: Mammalian Cell Engineering, Molecular Bio-processing, Targeted Gene Integration, Cell culture, CHO cells - Bacteria - Yeast - Mice- C. elegans models, Molecular biology, Protein Biochemistry, Recombinant protein Expression, Purification and Analysis, Analytical chemistry, Medical Microbiology, Immunology, Bio-Iron metabolism in Neuro-degenerative diseases, Anti microbial peptides, Microscopy and Sequencing
Senior Postdoctoral Scientist
Since November 2014
Mammalian Cell Engineering Molecular Bio-processing miRNA manipulation for Recombinant Protein Production
Post doctoral researcher
March 2012 --- March 2014
Neuronal and Systemic iron metabolism in normal and neurodegenerative diseases like (sCJD, AD and PD) and the role of Prion protein in Iron metabolism in transgenic mouse and cell culture models. Iron transport proteins, divalent metal transporters, Protein-Protein interactions, live cell imaging with florescent labelled proteins.
Post doctoral researcher
March 2011 --- February 2012
Bioengineering & biosynthesis of Thiostrepton A Quinaldic acid - gene disruption, gene expression, isolation of new metabolites, recombinant protein expression, purification and biochemical characterization.
October 2007 --- January 2011
Bioengineering of lantibiotic Lacticin 3147 - A two peptide lantibiotic investigated in to its molecular structure and functional analysis, charged residues,post translational modifications, lanthionines and D-alanine formation using mutagenesis approach for generating a Blue Print for Lantibiotic Drug Designing.
June 2007 --- September 2007
Cytokine, hormone & disease marker levels analysis to determine the effect of putative novel treatments in IBD
October 2006 --- September 2007
Identifying Modifiers of triplet nucleotide repeats in Caenorhabditis elegans. Gene silencing strategy used to study breast cancer related homologues for different phenotypes. RNAi, RT-PCR, gene sequencing.
CollaborationAdaptabilityCommunicativeCreative thinkingEfficiencyProblem solvingFlexibilityOptimismAuthenticity
Skills and Expertise
Analytical skills Develop protocols Guide students Interpret data Lab scale batches Molecular Diagnostics Report data Write papers Write protocolsAnalyze proteinCell culturecollaborate in research projects at universitiesCommunicate with investigatorCreate SOPsDesign protocolLead studiesPresent data at congressProvide trainingProvide technical supportR&D
Ph.D in Molecular Biology & Microbiology from University College Cork in 2010
M.Sc in Biotechnology from University College Cork in 2007
M.Sc in Medical Microbiology from Manipal University in 2002
B.SC in Biochemistry & Microbiology from Acharya Nagarjuna University in 1999