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Summary

Specialties: Mammalian Cell Engineering, Molecular Bio-processing, Targeted Gene Integration, Cell culture, CHO cells - Bacteria - Yeast - Mice- C. elegans models, Molecular biology, Protein Biochemistry, Recombinant protein Expression, Purification and Analysis, Analytical chemistry, Medical Microbiology, Immunology, Bio-Iron metabolism in Neuro-degenerative diseases, Anti microbial peptides, Microscopy and Sequencing

Experiences

Current Experience

  • Senior Postdoctoral Scientist
    Since November 2014

    Mammalian Cell Engineering Molecular Bio-processing miRNA manipulation for Recombinant Protein Production

Past Experience

  • Post doctoral researcher
    March 2012 --- March 2014
    Neuronal and Systemic iron metabolism in normal and neurodegenerative diseases like (sCJD, AD and PD) and the role of Prion protein in Iron metabolism in transgenic mouse and cell culture models. Iron transport proteins, divalent metal transporters, Protein-Protein interactions, live cell imaging with florescent labelled proteins.

  • Post doctoral researcher
    March 2011 --- February 2012
    Bioengineering & biosynthesis of Thiostrepton A Quinaldic acid - gene disruption, gene expression, isolation of new metabolites, recombinant protein expression, purification and biochemical characterization.

  • Research Assistant
    October 2007 --- January 2011
    Bioengineering of lantibiotic Lacticin 3147 - A two peptide lantibiotic investigated in to its molecular structure and functional analysis, charged residues,post translational modifications, lanthionines and D-alanine formation using mutagenesis approach for generating a Blue Print for Lantibiotic Drug Designing.

  • Research Assistant
    June 2007 --- September 2007
    Cytokine, hormone & disease marker levels analysis to determine the effect of putative novel treatments in IBD

  • Graduate student
    October 2006 --- September 2007
    Identifying Modifiers of triplet nucleotide repeats in Caenorhabditis elegans. Gene silencing strategy used to study breast cancer related homologues for different phenotypes. RNAi, RT-PCR, gene sequencing.

Personality

Self Assessment :
CollaborationAdaptabilityCommunicativeCreative thinkingEfficiencyProblem solvingFlexibilityOptimismAuthenticity

Knowledge

LinkedIn Assessment :
molecular biologyProtein ExpressionCell CultureRT-PCRBiochemistryPurificationCellPCRMolecular CloningMicrobiologyHPLCWestern BlottingMicroscopyProtein chemistryBioinformaticsqPCRBiotechnologyFlow CytometryFluorescence MicroscopyTransfectionMass SpectrometryImmunologyGel ElectrophoresisRNA isolationDNASDS-PAGEELISACell biologyFPLCUV/VisNMRRNAiConfocal MicroscopyAnalytical ChemistryProtein PurificationSequencingDNA extractionCell Based AssaysImmunofluorescenceC. elegansProtein SequencingLyophilizationSouthern BlotMutagenesisProtein-protein InteractionsmiRNAdroplet digital PCRCRISPR Gene TargetingCharacterization

Skills and Expertise

Self Assessment :
Analytical skills Develop protocols Guide students Interpret data Lab scale batches Molecular Diagnostics Report data Write papers Write protocolsAnalyze proteinCell culturecollaborate in research projects at universitiesCommunicate with investigatorCreate SOPsDesign protocolLead studiesPresent data at congressProvide trainingProvide technical supportR&D

Education

  • Ph.D in Molecular Biology & Microbiology from University College Cork in 2010
  • M.Sc in Biotechnology from University College Cork in 2007
  • M.Sc in Medical Microbiology from Manipal University in 2002
  • B.SC in Biochemistry & Microbiology from Acharya Nagarjuna University in 1999

Languages

BrightOwl Assessment:
Self Assessment:
English
Full Proficiency
TELUGU
Native
Hindi
Full Proficiency

Work Preferences

  • Notice Period:
    9 weeks
  • Positions I am interested in:
    Senior Scientist Scientist Biomedical Scientist
  • Locations I am interested in:
    Ireland UK USA
  • Work From Home:
    No
  • Work Regime:
    Permanent position
  • International:
    Yes

Area / Region

Dublin, Ireland

Others

Driving License
  • Yes