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Summary

Measuring, characterizing, quantifying and purifying accurately biomolecules such as proteins, nucleic acids or compounds such as antimicrobials may lead to better treatment possibilities and health status monitoring for the patients.

 

I am a biochemist with passion for Life Sciences, Biotechnology, Innovation and Quality.

 

I have completed my Master and Doctoral studies at EMBL (European Molecular Biology Laboratory), in France and then in Germany. There, I was working on biochemistry, protein purification, characterisation and structural biology. In 2008, I defended my PhD thesis on the functional and structural characterisation of the interaction between human and influenza virus proteins.

 

Then, I have been working as a post-doctoral fellow at the Joint Research Centre of the European Commission in Belgium. In this frame I was working in a research group that produces Certified Reference Materials for protein quantification to support standardisation in the In-Vitro Diagnostics. Aside the laboratory work I was in charge of, I was coordinating a large scale multiple analysis study involving several European laboratories. Thanks to this work under the ISO 17025 accreditation, I have improved my experience in working under a quality management system and in project management. I was as well the instrument responsible for the protein purification platforms (ÄKTA purifiers) and I used to give trainings for the authorisation of users within the group.

 

Currently, I am working at TOKU-E EU in Belgium as a scientific manager. The Company TOKU-E is specialised in the development and manufacturing of ultra-pure antibiotics and fine chemicals for life sciences research.

 

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Experiences

Current Experience

  • Scientific Manager
    Since August 2014

    Overall purpose: High-purity antibiotics Research and Development for biotechnology applications

    Main areas of expertise

    • Purification and characterisation of antibiotic compounds

    • High-grade compounds production

    • Minimum Inhibitory Concentration (MIC) value screening

    • Cell line toxicity effect screening

    • Quality System of the laboratory

     

    Management and organisation

    • Design and setting-up of a R&D laboratory

    • Strategic planning of the scientific work

    • Scaling-up of the R&D activities to a production facility

    • Prioritization of the needs of the laboratory

    • Negotiation of the equipment to be purchased

    • Involvement in the selection process of scientific staff

Past Experience

  • Protein Scientist
    June 2013 --- August 2013

    Short term contract to reinforce research activities within a microbiology team

    Overall purpose: Protein purification and protein characterisation in food safety

     

    Laboratory and scientific work

    • Design and carry out scientific research and laboratory experiments

    • Carry out protein measurements and quantification

    • Chromatography: size exclusion, ion exchange, affinity, ÄKTA systems

    • IgG purification using protein A method

    • Electrophoresis: SDS-PAGE

    • Immunoassay: Western blot

    • Write reports

     

    Management and organisation

    • Work under ISO Guide 34 and ISO 17025 accreditation

  • Postdoctoral Scientist / Scientific Project Officer
    November 2009 --- October 2012

    Overall purpose: Clinical research (characterisation & quantification of serum proteins) - Production of Certified Reference Materials - Instrument responsible and trainer for chromatography system (ÄKTA purifier)

     

    Laboratory and scientific work

    • Design and carry out scientific research and laboratory experiments

    • Carry out protein measurements and quantification

    • Chromatography: size exclusion, ion exchange, ÄKTA and Bio-Rad systems

    • Electrophoresis: SDS-PAGE, semi native and native gel

    • Immunoassay: Western blot, ELISA

    • Evaluate laboratory results including statistical analysis

    • Write reports and scientific publications

    • Method validation

    • Assist in certification campaigns

     

    Management and organisation

    • Work under ISO Guide 34 and ISO 17025 accreditation

    • Writing of SOPs

    • Coordinate a large scale multiple analysis study involving several European laboratories

    • Establish the work programme of the task (objectives, outputs, deadlines)

    • Prepare and maintain task and quality plans and track activities against the plan

    • Provide accurate reports

    • Monitor time scales and work progress, and take action where these deviate from agreed tolerances

    • Guide and follow up sub-contractors in charge of task activities and review their deliverables

    • Support the establishment of a new protein and biochemistry laboratory

  • Postdoctoral Visiting Scientist
    January 2009 --- September 2009

    Extension of my PhD thesis as a post-doctoral visiting scientist

    Topic: study of the interaction between the viral genome of influenza virus (vRNA) and its nucleoprotein

     

    • Protein expression and purification

    • Chromatography: size exclusion, affinity, ÄKTA systems

    • In vitro transcription • Negative stain electron microscopy

    • Biophysics: isothermal calorimetry (ITC), analytical ultracentrifugation

    • Scientific publications

  • PhD Scientist in Biochemistry & Structural Biology
    October 2005 --- December 2008

    Thesis completed under the supervision of Dr. Florence Baudin

    Topic: functional and structural characterisation of the interaction between human and influenza virus proteins

     

    • Expertise in protein expression, purification and crystallization

    • Biochemistry

    • Chromatography: size exclusion, ion exchange, affinity, ÄKTA and Bio-Rad systems

    • Cell culture (E. coli) • Electrophoresis: SDS-PAGE, native gel, agarose gel, urea-PAGE

    • Molecular biology: cloning, PCR, plasmid purification, E. coli transformation, in vitro transcription

    • Structural approach: crystallogenesis, crystallography, negative stain electron microscopy

    • Biophysics approach: circular dichroïsm, UV spectrometry

    • Bioinformatics tools: 3D data viewers, structure predictions, sequence alignments

    • Communication of results: scientific publications, oral presentations, poster presentations

    • Popularisation of science

Personality

Self Assessment :
Attention to detailCollaborationCompetitivenessCoordinationCuriosityOrganizationTrust

Knowledge

Self Assessment :
Biochemistry R&D Scientific writingBiological Drug DevelopmentBiomarkersBiotechnologyClinical researchDNAELISAIt skillsmolecular biologyNegotiationpeople managementProtein ExpressionVirology
LinkedIn Assessment :
Biochemistrymolecular biologyProtein PurificationProtein chemistryStatisticsLife SciencesProtein ExpressionscienceResearchLifesciencesChromatographyPCRCharacterizationGel ElectrophoresisISO 17025BiotechnologyLaboratoryCell CultureScience CommunicationImmunoassaysProject ManagementStructural BiologyScientific ManagementClinical researchData AnalysisMolecular CloningMulticultural EnvironmentsPublic SpeakingSDS-PAGEWestern Blotting

Skills and Expertise

Self Assessment :
Analyze data Create SOPs Develop protocolsAnalyze proteinAssign activitiesEvaluate impurity identificationExecute scientific projectslifescienceNegotiationNetworkSite management

Education

  • Doctor of Philosophy (PhD) in Biochemistry - Structural Biology from Université Claude Bernard Lyon 1 in 2008
  • MASTER in Structural Biology & Nanobiology from Université Joseph Fourier (Grenoble I) in 2005
  • MASTER'S DEGREE in Life Science from Université Joseph Fourier (Grenoble I) in 2004
  • BACHELOR’S DEGREE in Biochemistry from Université Joseph Fourier (Grenoble I) in 2003

Languages

BrightOwl Assessment:
Self Assessment:
French
Native
English
Full Proficiency
Dutch
Elementary Proficiency
Spanish
Elementary Proficiency

Area / Region

Gent, Gand, Belgique

Others

Driving License
  • Yes