Analytical thinkingAttention to detailCuriosityProactivityOrganizationAdaptabilityAuthenticityInterest in knowledgeIndependenceProblem solving
molecular biologyImmunologyCell biologyNeurobiologyGPCRCel adhesion moleculesImaging TechnologiesProtein BiochemistrypharmacologyNeuron-Glia interactionsSignal Transduction Virology
Skills and Expertise
TECHNICAL EXPERTISE- Implementation Execution Problem-solving Analysis Interpretation and Control of scientific processes according to the recognized standards and GLP guidelines in the following areas: • Molecular Biology subcloning and mutagenesis by PCR sequencing analysis transcription in vitro DNA/RNA extraction (RT-)qPCR gene silencing (i.e. by siRNA)/overexpression with recombinant plasmid or viral vectors • Cell Biology excellent mastering of aseptic technique for cell culture in a BSL2- or BSL3-environnement of immortalized cell lines and primary cultures from human adipose tissue epithelial cells from mouse choroid plexus neurons from mouse dorsal root ganglion or cortex organotypic hippocampal slice culture. Transient protein expression by electroporation lipotransfection or viral infection following of this expression by confocal microscopy and videomicroscopy; cytotoxicity assays; paraffin processing of tissue • Microscopy and imaging from the use of the first-generation confocal microscope (Leica) the 2-photon confocal microscope the Zeiss apotome-attached fluorescent microscope to the Zeiss LSM780 Laser Confocal microscope. Dynamic FRAP experiments. Proficiency in the use of Metamorph for dynamic calcium imaging and the use of different phosphoimagers. • Pharmacology pharmacological characterization of HIV-1 protease inhibitors and antidepressants IP3 measure Ca2+measure by imaging or automated multireader ELISA radioimmunoassays • Biochemistry and Proteomic recombinant protein production and purification by affinity chromatography pull-down and (co-)immunoprecipitation assays Western Blot 2D-Electrophoresis MALDI-TOF mass spectrometry peptide-array surface plasmon resonance spectroscopy (UV fluorescence) COMPUTER SKILLS- Proficient use of Internet Microsoft Office programs image processing and analyzing softwares such as Adobe Photoshop Metamorph Zen ImageJ Molecular Biology softwares GraphPad statistical software. Fearlessness to learn and manage EQMS and other informatic platforms.
Ph.D. in Health-Biology, specialty in Integrative Neurosciences from CBS2 Doctoral School- Montpellier Medicine University (UM1) in 2008
Master in Biochemistry and Molecular Biology, specialty in Microbiology and Virology from Denis Diderot University (Paris VII) in 2004
Expert has 10 publications (Will be avalible with full profile)