I am Antoine Hubert, I have a formation of cellular and molecular biologist, associated with proteomics and lipidomics expertise.
Benevolat Leuven, Belgium
Since December 2014
Articles last issues to fix
Academic staff Leuven, Belgium
December 2011 --- December 2014
Post-doc in Proteomics and lipidomics to determine the existence of subpopulations of exosomes in Lim1215 colon cancer cells.
Other projects handeled:
- Discovery of new biomarkers in endometriosis.
- Proteomic characterization of the Baboon model for endometriosis.
- Research of "missing genes" in Pseudopodoces humilis.
- Studies of new interacting partners of Cip2.
- Discovery of new molecules of interest in Brasil's originating spider venom.
Academic staff Leuven, Belgium
December 2007 --- December 2011
PhD in cancer fundamental research (biomedical sciences), with establishment of stable inducible cell lines (TET-ON system), dual-tag purification of the protein of interest, followed by proteomics. Proteomics are consisting in 1D and 2D electrophoresis (2D DIGE), followed by MALDI-TOF-TOF and Mascot search to identify interactors of the protein of interest. The following validation of some of the interactions was done by CoIP. Biological relevance checked by confocal microscopy.
Stagiaire Liège, Belgium
January 2005 --- June 2005
Optimization of Paraffin embedding protocols in Dyax Corp, optimization of migration and invasion assays, cryostat slicing
Analytical thinkingInnovative thinkingInterest in knowledgeKindnessOptimismPerspectiveProblem solvingTrustWillingness to compromiseFlexibilityEfficiencyCuriosityAttention to detailAuthenticityCharmCollaborationCompetitivenessCoordinationCreative thinkingCritical thinkingAdaptability
Biochemistry Cell biology Lab scale batches R&D Scientific writing LabtechnicianAntibodiesBiomarkersBiotechnologyCancerCell biologyCell CultureClinical researchConfocal MicroscopyData AnalysisData cleaningData ManagementDNA extractionDNA sequencingELISAEnglishEnzyme-linked immunosorbent assay (ELISA)Flow CytometryFluorescence MicroscopyFluorescent microscopyImmunofluorescenceImmunohistochemistry (IHC)ImmunoprecipitationIn VitroIn VivoLaboratoryLabtechnicianMicroscopyMicrosoft ExcelMicrosoft OfficeMolecular & Cellular Biologymolecular biologyMolecular CloningPCRpeople managementPharmaceutical IndustryPhenotype PowerPointProblem-solving methods and troubleshootingProcess improvementProject ManagementProtein ExpressionProtocolPrototyping techniquesR&DRT-PCRScientific methodologyScientific WritingSDS-PAGEFlow CytometryWestern BlottingHPLC
Skills and Expertise
Analyze data Control data Develop protocols Guide students Interact with physicians Interact with nurses Interpret data Lab scale batches Report data Search literature on clinical trials Technology research Use a confocal microscopy Write papersAdjust methodsAdjust processes Analyze proteinAssist with experimentsAssist with routine testsAssist with proceduresAttend seminarsBiomarker ResearchCollaborate with medical teamCollaborate with principal investigatorCollaborate with project teamConfocal microscopyControl protocol versionsData analysisData miningData validationData verificationDesign protocolDirect co-workersEnsure data consistencyEnsure data integrityEvaluate protocolsInteract with pre-clinical scientistsInterpret datamanaging a small teamManuscript preparationManuscript preparation and reviewOral presentationPresent data at congressProvide trainingShipment of biological specimenValidate dataVerify dataUse western blotting techniqueWritten presentation
Master in Cellular and Molecular Biology from FUNDP in 2005
Bachelor in Cellular and molecular Biology from FUNDP in 2004
Training and Certification
Msc in 2005 Certification