after obtaining my medical degree, i have been working in molecular Cell biology for over 15 years, with 10 years of experience with Stem Cells and protein analysis, as well as experience with cell-based Assays, Immunology Assays, Good Laboratory Practice (glp), good Documentation practice (gdp) and Quality Management System ISO 9001:2008. innovative Scientist, applying knowledge to optimize protocols and develop new strategies and practical solutions to issues. hands-on team player, ensuring excellence in Research, Project Management and Testing process.
areas of expertise include:
molecular biology techniques · Cell biology · cell isolation, culture, manipulation and Characterization · cell-based Assays · viral vectors · Transfection/transduction of cells · RNA-based Biomarker Assays · Immunology Assays · ELISA · Flow Cytometry · facs · Biomarker investigation · Protein Expression, Purification and analysis · HPLC · strategic analysis/Planning · Project Management · Problem Solving · methodology development · Statistical Data Analysis · Translational Medicine
Research fellowDavos, Switzerland
Since January 2014
lead Research on development of a reliable cell-based assay to monitor live Stem cell differentiation for drug screening using Stem Cells, 3d Cell Culture, co-culture system, viral vectors, fluorescence/Luminescence-based cell reporter Assays, RNA-based Biomarker assay, Flow Cytometry, facs, Immunocytochemistry, Fluorescence microscope, ELISA, DNA modification and cloning, qPCR and image Analysis. design and perform experiments according to glp, gdp and ISO 9001:2008.
Lead research on development of a reliable cell-based assay to monitor live stem cell differentiation for drug screening using stem cells, 3D cell culture, co-culture system, viral vectors, fluorescence/luminescence-based cell reporter assays, RNA-based biomarker assay, flow cytometry, FACS, immunocytochemistry, fluorescence microscope, ELISA, DNA modification and cloning, qPCR and image analysis. Design and perform experiments according to GLP, GDP and ISO 9001:2008.
Postdoctoral Research Fellow Zürich, SwitzerlandJanuary 2013 --- December 2013
led major study on influence of ecm Proteins on Stem Cells using Cell-based Assays and Immunology Assays. isolated and characterized ecm Proteins using HPLC, Gel Electrophoresis, Western blot, circular dichroism (cd), Fluorescence resonance Energy transfer (fret) and Confocal Microscopy.
Research Assistant Beijing, ChinaSeptember 1998 --- June 2002
focused on expression, Purification and Characterization of recombinant Proteins for diagnosis of nasopharyngeal carcinoma using expression vectors, e.coil, cho cells (pcmv and Mammalian Cell expression system), sf9 cells (baculovirus expression vector and insect Cell expression system), stirred tank bioreactor, HPLC and ELISA.
Innovative thinkingProblem solvingAnalytical thinkingCritical thinkingIndependenceCollaboration
Cell Culture Cell biologyCell Based AssaysStem cell researchmolecular biologyImmunoassaysBiomarkersELISAHPLC BiochemistryProtein ExpressionProtein PurificationFlow CytometryFluorescence MicroscopyConfocal Microscopy BiostatisticsViral VectorsProject ManagementScientific methodology Animal modelsMolecular Cloning
Skills and Expertise
Analyze dataCell cultureOptimize assay performanceAnalyze proteinTranslate strategy into operational plans Molecular DiagnosticsBiomarker Research Use a confocal microscopyUse fluorescence microscopyDesign the study Analytical skillsInterpret scientific dataPresent data at congressLead studiesSet up research projectsGuide students
Doctor of Philosophy (Ph.D.) in Biomedical Sciences from Eidgenössische Technische Hochschule Zürich in 2012
Master of Science (M.Sc.) in Microbiology and Immunology from National University of Singapore in 2005
Bachelor of Medicine, Bachelor of Surgery (M.B.B.S.) in Clinical medicine from Capital University of Medical Sciences in 1998
Training and Certification
Practical Statistics for Biology/Medical Research in 2016 Training
Animal Experiment Coursework in 2005 Certification
Expert has 2 publications (Will be avalible with full profile)