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Summary

Publications and citations - http://scholar.google.nl/citations?user=9qp5tleaaaaj&hl=en i have a broad background ranging from molecular biology to microbial Physiology and industrial Biotechnology, as obtained during my bachelors, masters and phd. during by bachelors from ggsipu, i was introduced with many aspects of Biotechnology along with the hands on experiences of general techniques. my masters Education exposed me to the field of bio-process technology and Bioengineering with major emphasis on Applications of thermosensitive Polymers. during my phd-project at University of amsterdam, i specialized in Fermentation technology, microbial Physiology, Cell Culture and Analysis techniques and Project Management. my project broadly dealt with the bacterial respiration and focused on the electron transport chain of e. coli, its component and their interactions. additionally, i have also worked on the In Vitro meat production project, where i was mainly dealing with the large scale optimization of Mammalian cell growth in terms of efficiency and stability under the continuous culture conditions, followed by metabolomics. specialties:industrial Biotechnology, microbial Physiology, Bioengineering, molecular biology, Cell Culture

Experiences

Current Experience

  • Researcher Microbiology


    Since June 2014

Past Experience

  • Helpdesk and Communications/Publications Volunteer

    January 2014 --- May 2014
    http://www.calameo.com/read/001197593f79cc123e802

  • Writer - Dutchness - The Foreign Perspective

    January 2014 --- May 2014
    http://dutchreview.com/author/poonam-sharma/

  • Graduate student

    July 2009 --- November 2013
    phd thesis “on the efficiency of glucose catabolism in organisms ranging from e. coli to man” supervised by prof. dr. klaas jan hellingwerf and prof. dr. m. (joost) teixeira de mattos the project broadly deals with the respiration in e.coli and optimization of growth in Mammalian Cell Culture with emphasis on topics listed below: • role of demethyl-menaquinone in aerobic respiration • functionality of cytochrome oxidase bd-ii • contribution of “uncoupling pathways” in the catabolism of glucose in e. coli • role of quinones in arcba-mediated regulation of the aerobic/anaerobic transition in e.coli • optimization of the growth efficiency of chinese hamster ovary (cho) cells in defined medium • metabolomics of chemostat-grown cho cells with newly developed techniques

  • Masters Project

    January 2008 --- May 2009
    master’s thesis “application of smart Polymers” supervised by prof. dr. ashok kumar the m.tech project revolved around a few Applications of smart Polymers as listed below: • Bioengineering Applications of thermoresponsive Polymer for Protein Purification • role of thermoresponsive Polymer in hepatocytes spheroid formation • synthesis of protein-Polymer bioconjugates • inclusion bodies purification and refolding using cryogels

  • Bachelor's Project

    January 2007 --- May 2007
    bachelor’s thesis “downstream metabolites effect on nitrogen assimilation pathway” supervised by dr. n. raghuram the dissertation project was focused on the effects of downstream metabolites of nitrogen assimilation on nitrate reductase and nitrite reductase in oryza sativa.

  • Intern

    June 2006 --- July 2006
    “computation Analysis of DNA and Protein bands” supervised by dr. j.s. virdi the summer project was entitled “computer assisted Analysis of DNA and Protein fingerprinting data of yersinia enterocolitica isolated from india”

Knowledge

LinkedIn Assessment :
BioinformaticsBiochemistryLifesciencesmolecular biologyCell CultureMicrobiologyFood MicrobiologyFermentation TechnologyDownstream processingBioengineeringProcesses DevelopmentProject ManagementBioprocessingBiomaterialsWestern BlottingProtein PurificationBiotechnologyMammalian Cell CultureIn VitroSystems BiologyPurificationDNAProtein chemistrySDS-PAGEGeneticsMicroscopyELISAFermentationGel ElectrophoresisChromatographyMetabolomicsTissue CultureHPLCEnzyme AssaysEnzyme Kinetics

Education

  • Doctor of Philosophy (PhD) in Microbial physiology and molecular biology from University of Amsterdam in 2013
  • Master of Technology (M.Tech.) in Biological sciences and bioengineering from IIT Kanpur in 2009
  • Bachelor of Technology (B.Tech.) in Biotechnology from Guru Gobind Singh Indraprastha University in 2007
  • Schooling in from Kendriya Vidyalaya in 2002

Area / Region

Netherlands

Others

Driving License
  • No

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