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Summary

good scientific, Communication and computer skills team spirit, open-minded self-motivated, perseverant, rigorous and organised worker

Experiences

Current Experience

  • R&D Scientist - Molecular Biologist


    Since May 2010
    • cloning in adenovirus, baculovirus and e.coli vectors, Transfection (sf9, 293a cells), transformation of Bacteria by heat shock, Cell lysis (sonication, disruption, chemical), culture of aerobic and anaerobic Bacteria, RNA-DNA extractions from various tissues (feces, Virus, lungs, spleens) using column or magnetic beads from commercial kits, real time (rt)-PCR, Diagnostic PCR, production and Purification of recombinant Proteins by imac, ions exchange or affinity Chromatography (glutathion, s-Protein columns), Antibodies Purification, SDS-PAGE Electrophoresis on phastsystem (coomassie blue and silver staining), western-blotting, diafiltration (stirred Cell), cleavage of Protein using proteases • real time (rt)-PCR : development of commercial Diagnostic kits (duplex or triplex) with 7500 fast system, Diagnostic Assays, taqman probes (mgb/bhq-1) and primers design with primerexpress. • achievemnent and development of antigenic and serological ELISA tests (direct, indirect, competition). Immunofluorescence Assays. • development of DNA microarrays • familiar with ISO 9001 standards and glp's – use of 4th dimension (4d) Software • writing of scientific reports • supervision of master students or interns • Cloning in adenovirus, baculovirus and E.coli vectors, transfection (sf9, 293A cells), transformation of bacteria by heat shock, cell lysis (sonication, disruption, chemical), culture of aerobic and anaerobic bacteria, RNA-DNA extractions from various tissues (feces, virus, lungs, spleens) using column or magnetic beads from commercial kits, Real Time (RT)-PCR, diagnostic PCR, production and purification of recombinant proteins by IMAC, ions exchange or affinity chromatography (glutathion, S-protein columns), antibodies purification, SDS-PAGE electrophoresis on PhastSystem (Coomassie blue and silver staining), Western-blotting, diafiltration (stirred cell), cleavage of protein using proteases • Real Time (RT)-PCR : Development of commercial diagnostic kits (duplex or triplex) with 7500 Fast System, diagnostic assays, TaqMan probes (MGB/BHQ-1) and primers design with PrimerExpress. • Achievemnent and development of antigenic and serological ELISA tests (direct, indirect, competition). Immunofluorescence assays. • Development of DNA microarrays • Familiar with ISO 9001 standards and GLP's – use of 4th Dimension (4D) software • Writing of scientific reports • Supervision of Master students or interns

Past Experience

  • PhD in Cellular and Molecular Biology

    September 2005 --- May 2010
    • general molecular biology techniques: PCR (gradient), cloning in e.coli, library construction (random cassettes), directed mutagenesis, transformation by electroporation, bacterial cultures (tg1, bl21(de3)…) in liquid or solid medium, expression and Purification of Proteins (affinity Chromatography, ions exchange, imac with Äkta system), dialysis, etoh precipitation, phenol-chloroform extraction, centrifugation • Enzyme Characterization: measures of enzymatic activity, kinetic parameters determination, measures of Protein stability (gu.hcl) • general Analytical techniques: SDS-PAGE, agarose Gel Electrophoresis, Western Blotting, Sequence Analysis (bioedit, vector nti softwares) • phage display: filamentous bacteriophages production, Purification and affinity selection (library screening) • writing of scientific papers, • scientific conferences participations • supervision of master students • universitary workshops preparation

Knowledge

LinkedIn Assessment :
Biologie molRTransfert de protELISASciences de la vieCelluleSDS-PAGEADNPurificationRT-PCRRNAR&DTransfection

Education

  • Ph.D in Science - Cellular and Molecular Biology from Université catholique de Louvain in 2010
  • DEA in Science - Cellular and Molecular Biology from Université catholique de Louvain in 2007
  • Master in Chemical Science in Chemistry from Université catholique de Louvain in 2005
  • in from Collège Saint-Augustin, Gerpinnes in 2001

Area / Region

Namur, Belgium

Others

Driving License
  • Yes

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