Research AssistantBrussels, Belgium
Since March 2014
antibiotic resistance has become a worldwide problem that threatens the effectiveness of many medicines used today to treat bacterial infections. a particularly serious threat is the emergence of gram-negative pathogens, including escherichia coli, which are resistant to all of the available antibacterial agents.it is therefore urgent to develop new antibiotics active against gram-negative Bacteria, which requires a deep understanding of the Biology of these organisms, and particularly of the complex networks involved in bacterial envelope biogenesis and integrity. the envelope of gram-negative Bacteria is a complex macromolecular structure that serves as the first barrier to environmental stresses (eg. antibiotics). this resistance is due to complicated signaling systems that modulate the expression of specific genes. several envelope stress response systems have been described in the bacterial model e. coli (rcs system, cpx system…) but the functioning of some of them is poorly understood. following the evolutionary path of specific components of these networks in several bacterial models should provide important information about the antibiotic resistance acquisition in e. coli. combining both evolutionary Analysis (phylogenomics, comparative Genomics, co-occurrence…) and experimental approach could lead to the identification of new therapeutic targets.
Antibiotic resistance has become a worldwide problem that threatens the effectiveness of many medicines used today to treat bacterial infections. A particularly serious threat is the emergence of Gram-negative pathogens, including Escherichia coli, which are resistant to all of the available antibacterial agents.It is therefore urgent to develop new antibiotics active against Gram-negative bacteria, which requires a deep understanding of the biology of these organisms, and particularly of the complex networks involved in bacterial envelope biogenesis and integrity. The envelope of Gram-negative bacteria is a complex macromolecular structure that serves as the first barrier to environmental stresses (eg. antibiotics). This resistance is due to complicated signaling systems that modulate the expression of specific genes. Several envelope stress response systems have been described in the bacterial model E. coli (Rcs system, Cpx system…) but the functioning of some of them is poorly understood. Following the evolutionary path of specific components of these networks in several bacterial models should provide important information about the antibiotic resistance acquisition in E. coli. Combining both evolutionary analysis (phylogenomics, comparative genomics, co-occurrence…) and experimental approach could lead to the identification of new therapeutic targets.
Post-doctoral researcher Marseille, FranceFebruary 2010 --- January 2014
myxococcus xanthus utilizes two distinct motility systems for movement on solid surfaces: adventurous motility (a-motility) for individual Cell movement and social motility (s-motility) for coordinated group movements. both systems have two genetically, functionally and structurally different motility engines with two distinct and different evolutionary histories. nevertheless, motility is essential for m. xanthus to accomplish complex social behavior (fruiting bodies formation, predation) and this it has evolved a regulatory system to coordinate the motility engines. here, based on the knowledge that the regulation of a and s-motility is operated by a set of chemotaxis-like Proteins encoded by the frz operon, we used a phylogenetic and comparative Genomics approach to investigate how coordination evolved.
our investigation showed that frz is a specialized chemosensory-like system that expanded through the recent duplication, fusion and recruitment of new genes. initially this system has been probably dedicated to the regulation of type iv pili involved in twitching. its expansion occurred in parallel to the development of gliding machinery in m. xanthus and more generally in myxococcales. the new components of the frz system interact specifically with a- and/or s-machinery allowing the coordination of both motilities machinery. on the basis of our results we propose an evolutionary model that traces the establishment of coordination between the two systems of motility in m. xanthus. experimental approaches to test the computational predictions will also be presented.
AdaptabilityAttention to detailCollaborationCuriosityOrganizationResponsibilitySociabilityResult OrientedReaction to stress
Biochemistry R&D Negotiation Scientific writingBioinformaticsBiotechnologyChromatographyDNADNA extractionEnglishGel ElectrophoresisGeneticsGenomicsHPLCIn VitroIn VivoLaboratoryLaboratory ResearchLaboratory TechniquesLaboratory testingMicrobiological TechniquesMicrobiologyMicrosoft PowerpointMicrosoft OfficeMicrosoft ExcelMolecular & Cellular Biologymolecular biologyMolecular CloningMolecular geneticsOxidative stressPatentsPCRPowerPointProject ManagementProtein ExpressionProtein PurificationProtocolR&DReport WritingScientific methodologyScientific WritingSDS-PAGESterilizationTeam LeadershipWestern BlottingGMPBacteriologyBiologyBiotechnologieBiomedical SciencesElectrophoresisTeam ManagementTeachingscienceScientific CommunicationsResearchR&DPurificationProteinsProtein EngineeringPresentationsLinuxLaboratory ManagementLaboratory SkillsEnglishValidation
Skills and Expertise
Develop protocols Analyze data Guide students Molecular Diagnostics Report data Technology research Write papersAdjust methodsAdministrative supportAnalyze proteinArchive documentationArchive study documentsAssist with experimentsAssist with routine testsAttend seminarsConduct literature searchesConduct research at universitiesConduct university research Data analysisDesign protocolDesign scientific projects (in biology)Evaluate protocolsExecute scientific projectsGeneral office managementInterpret dataLiaise with research teamlifescienceManage laboratory proceduresManuscript preparationManuscript reviewOral presentationOrganize scientific projects (in biology)Present data at congressProtocol managementPublication of articlesPublish scientific projectsReport dataResearch at universitiesResolves queriesReview protocolsReview study protocolsSupervise PhD studentsSupervise techniciansUse western blotting techniqueUnderstand protocolsValidate dataVerify dataWork under specific instructionsWrite papersWrite protocolsWritten presentation
Ph.D. in Bioengineering from National School of Engineering - Sfax (Tunisia) in 2009
diploma in Protein Biochemistry from Pasteur Institute Paris (France) in 2008
Advanced Master in Molecular Medicine from Faculty of Medicine - Sfax (Tunisia) in 2006
Master in Bioengineering from National School of Engineering - Sfax (Tunisia) in 2004
Bachelor in Molecular and Cellular Biology from , Faculty of Science - Sfax (Tunisia) in 2002
Training and Certification
Introduction to programming using Python in 2012 Training
in over production of bacterial proteases in cell free system in 2008 Training
writing and submitting patents in 2006 Training
Quality Production (GMP, GLP, validation) in 2016 Certification
Expert has 17 publications (Will be avalible with full profile)