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Summary

a highly motivated and enthusiastic principal Scientist with a strong background in Mammalian Cell line development (cld) for the production of mabs. experienced in managing Innovation projects from the initial Planning and designing to a successful outcome with the aim of continuous improvement. experience of cld for commercial projects.

fluent English speaker.

Experiences

Current Experience

  • Principal Scientist

    United Kingdom
    Since December 2014

    lead on customer projects: establishment of transfected pools or clonal Cell lines for the production of recombinant Proteins (mabs) for external customers, meeting objectives/timelines

    • Communication with customers: writing reports, Presentations at teleconferences
    • collaboration with analytical and downstream process departments

     establishment of pro-forma for clonal Cell line traceability, writing up of work instructions and protocols

     responsible for populating a new coshh and risk assessments database for the department

    Lead on customer projects: Establishment of transfected pools or clonal cell lines for the production of recombinant proteins (mAbs) for external customers, meeting objectives/timelines

    • communication with customers: writing reports, presentations at teleconferences
    • collaboration with Analytical and Downstream process Departments

     Establishment of pro-forma for clonal cell line traceability, Writing up of work instructions and protocols

     Responsible for populating a new COSHH and Risk Assessments database for the Department

Past Experience

  • Senior Research Scientist United Kingdom

    July 2013 --- November 2014

    responsibilities within the establishment of apollotm: a cho Cell line development platform (animal component free):

    • vector construction and selection system
    • state of the art technologies (clonepix, facs, limiting dilution cloning, deep multiwell plates, tube spins, ambr15, rocking bioreactors)
    • Analytics: nova, ysi, octet, ELISA
    • patent search and collaboration with Patent Attorney

    establishment of clonal Cell lines with well known mabs to exemplify the new Cell line development platform

    • from vector construction to stable and high producing clonal Cell lines
    • Research Cell bank (rcb) and stability study
    • compiled confidential and non-confidential Marketing information (brochures, presentation slides, white papers)

    establishment of a pool expression system for rapid production of mabs, in small scales (from 15 ml up to 50 l in rocking bioreactors)

    actively involved in Laboratory organisation and improvement: 5S implementation

    experience in Student interviews for work placement

  • Research associate United Kingdom

    December 2008 --- November 2011

    responsible for the conduction of Research projects related to RNA biology.

    • planned, developed, conducted and analysed experiments, meeting objectives and timelines
    • Cell and molecular biology: cell lines and human primary cell cultures, transfections for Gene expression and knock-down experiments, DNA cloning, RNA extraction, rt-qPCR, northern blot, luciferase Assays
    • developed skills in Statistics and Microarray Data Analysis
    • experience in managing multiple scientific projects and collaborations with other labs
    • experience in managing junior colleagues: supervised undergraduate and phd students

    responsible for the development of a functional assay, for High Throughput Screening for a new class of anti-inflammatory drugs and for the exploration of its commercial potential.

    • developed and conducted the assay in human primary airway smooth muscle cells
    • developed a commercialisation plan for the technology
    • compiled non-confidential information and designed a Marketing brochure
    • contacted potential funders: attended conferences to publicise the technology

  • Research internship United Kingdom

    July 2007 --- February 2008

    responsible for the development and optimisation of a science)" rel="nofollow">Protocol of transient Transfection in suspension adapted cho cells, using pei, to produce recombinant his-tagged Proteins.

  • Research internship France

    July 2006 --- August 2006

    development of a Cell-based assay: a fluorescent biosensor of the apoptosis using Flow Cytometry (facs), for High Throughput Screening

    • stable Transfection of hela cells, selection of stable clones
    • vector construction, Bacteria transformation, mini and maxi prep, agarose gel

Personality

Self Assessment :
OrganizationResult OrientedCollaborationIndependenceTrustProactivityProblem solvingFlexibility

Knowledge

Self Assessment :
Mammalian Cell Cultureproduction of recombinant proteinsmolecular biologycell line developmentVector CloningEHSCOSHH and Risk assessment

Skills and Expertise

Self Assessment :
interact with custumersinteract with suppliersinteract with other departmentsehs advocateinteract with patent attorneyData analysis Guide studentslean six sigmayellow beltlab orgamisation (5S)

Education

  • Master in Biotechnology from ENSTBB in 2008

Languages

BrightOwl Assessment:
Self Assessment:
French
Native
English
Full Proficiency

Work Preferences

  • Notice Period:
    12 weeks
  • Locations I am interested in:
    Belgium
  • Work From Home:
    No
  • Work Regime:
    Permanent position
  • International:
    Yes

Area / Region

United Kingdom

Others

Driving License
  • Yes

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