BrightOwl Loader Loading


A highly motivated and enthusiastic Principal Scientist with a strong background in Mammalian Cell Line Development (CLD) for the production of mAbs. Experienced in managing innovation projects from the initial planning and designing to a successful outcome with the aim of continuous improvement. Experience of CLD for commercial projects.

Fluent English speaker.


Current Experience

  • Principal Scientist United Kingdom
    Since December 2014

    Lead on customer projects: Establishment of transfected pools or clonal cell lines for the production of recombinant proteins (mAbs) for external customers, meeting objectives/timelines

    • communication with customers: writing reports, presentations at teleconferences
    • collaboration with Analytical and Downstream process Departments

     Establishment of pro-forma for clonal cell line traceability, Writing up of work instructions and protocols

     Responsible for populating a new COSHH and Risk Assessments database for the Department

Past Experience

  • Senior Research Scientist United Kingdom
    July 2013 --- November 2014

    Responsibilities within the establishment of APOLLOTM: a CHO cell line development platform (animal component free):

    • vector construction and selection system
    • state of the art technologies (Clonepix, FACS, limiting dilution cloning, deep multiwell plates, tube spins, ambr15, rocking bioreactors)
    • analytics: NOVA, YSI, Octet, ELISA
    • patent search and collaboration with patent attorney

    Establishment of clonal cell lines with well known mAbs to exemplify the new cell line development platform

    • from vector construction to stable and high producing clonal cell lines
    • Research Cell Bank (RCB) and stability study
    • compiled confidential and non-confidential marketing information (brochures, presentation slides, white papers)

    Establishment of a pool expression system for rapid production of mAbs, in small scales (from 15 mL up to 50 L in rocking bioreactors)

    Actively involved in laboratory organisation and improvement: 5S implementation

    Experience in student interviews for work placement

  • Research associate United Kingdom
    December 2008 --- November 2011

    Responsible for the conduction of research projects related to RNA biology.

    • planned, developed, conducted and analysed experiments, meeting objectives and timelines
    • cell and molecular biology: cell lines and human primary cell cultures, transfections for gene expression and knock-down experiments, DNA cloning, RNA extraction, RT-qPCR, Northern blot, luciferase assays
    • developed skills in statistics and microarray data analysis
    • experience in managing multiple scientific projects and collaborations with other labs
    • experience in managing junior colleagues: supervised undergraduate and PhD students

    Responsible for the development of a functional assay, for High Throughput Screening for a new class of anti-inflammatory drugs and for the exploration of its commercial potential.

    • developed and conducted the assay in human primary airway smooth muscle cells
    • developed a commercialisation plan for the technology
    • compiled non-confidential information and designed a marketing brochure
    • contacted potential funders: attended conferences to publicise the technology

  • Research internship United Kingdom
    July 2007 --- February 2008

    Responsible for the development and optimisation of a protocol of transient transfection in suspension adapted CHO cells, using PEI, to produce recombinant His-tagged proteins.

    • optimisation of transfection : Design of Experiment and Flow Cytometry
    • scale up: 500ml spinner
    • His-tagged protein purification and analysis: IMAC, AKTA systems, ELISA, Enhanced ChemiLuminescence Western-Blot
    • developed ability to work independently: minimal supervision

  • Research internship France
    July 2006 --- August 2006

    Development of a cell-based assay: a fluorescent biosensor of the apoptosis using Flow Cytometry (FACS), for High Throughput Screening

    • stable transfection of HeLa cells, selection of stable clones
    • vector construction, bacteria transformation, mini and maxi prep, agarose gel


Self Assessment :
OrganizationResult OrientedCollaborationIndependenceTrustProactivityProblem solvingFlexibility


Self Assessment :
Mammalian Cell Cultureproduction of recombinant proteinsmolecular biologycell line developmentVector CloningEHSCOSHH and Risk assessment

Skills and Expertise

Self Assessment :
interact with custumersinteract with suppliersinteract with other departmentsehs advocateinteract with patent attorneyData analysis Guide studentslean six sigmayellow beltlab orgamisation (5S)


  • Master in Biotechnology from ENSTBB in 2008


BrightOwl Assessment:
Self Assessment:
Full Proficiency

Work Preferences

  • Notice Period:
    12 weeks
  • Locations I am interested in:
  • Work From Home:
  • Work Regime:
    Permanent position
  • International:

Area / Region

United Kingdom


Driving License
  • Yes