I have a M.Sc. degree in Biotechnology, with specialization in bioprocess engineering, from Lund University in Sweden. Between 2005 and 2014 I was involved in the development of new yeast strains for the production of bioethanol, at the Division of Applied Microbiology at Lund University (www.lth.se/lu_biofuels). I defended my Ph.D. thesis in May 2013 which, in short, became an evaluation of the status of the cellular processes required for cell proliferation under anaerobic conditions, with particular focus on the utilization of xylose as the carbon source. During my Ph.D. I obtained deep knowledge and insights of the metabolism, physiology and genetic engineering of Saccharomyces cerevisiae. Currently I am working on the development of industrial yeast platform strains in the Applied Metabolic Engineering group at the Novo Nordisk Foundation Center for Biosustainability in Denmark (www.biosustain.dtu.dk).
Since January 2014
October 2013 --- January 2014
Development of novel engineering strategies for efficient production of dicarboxylic acids from glucose and xylose using Saccharomyces cerevisiae.
March 2008 --- May 2013
Development of recombinant strains of Saccharomyces cerevisiae for efficient conversion of xylose to bioethanol with special focus on improving anaerobic growth on xylose. * Used kinetic models and computer simulations to identify potential limiting steps in the initial xylose pathways. * Used LC-MS/MS to capture the dynamic changes in intracellular metabolites during fermentation of a glucose/xylose mixture. Experiments were performed at ETH Zürich, Switzerland, in the lab of Prof. Uwe Sauer. * Used anNET to validate quantitative metabolite data through thermodynamic analyses and statistical tools (PCA, clustering) to extract biologically relevant information. * Evaluated the xylose-induced cAMP and trehalase responses in three different yeasts: Scheffersomyces stipitis, Candida tropicalis and Saccharomyces cerevisiae. Experiments were performed at KU Lueven, Belgium, in the lab of Prof. Johan Thevelein. * Evaluated the physiological effect of over-expressing compartment-specific components of the protein folding mechanism in recombinant yeast strains during xylose fermentation. * Generated a mutant library of Hxk2p using protein engineering techniques and identified a novel variant by combining genetic engineering and fermentation technology for efficient library screening.
January 2007 --- March 2008
Kinetic characterization of the enzymes required for xylose and arabinose utilization in Saccharomyces cerevisiae. * Used a ligation independent cloning (LIC) system for rapid heterologous gene expression in Escherichia coli. * Optimized the conditions for producing and purifying His6-tagged recombinant proteins. * Performed a comprehensive survey of kinetic rate equations and kinetic parameters for the construction of a kinetic model of yeast glycolysis.
Analytical thinkingSelf-confidenceKindnessSelf-disciplineInterest in knowledgeProblem solvingCuriosityTrustApproachabilityCritical thinkingResiliency
Skills and Expertise
Analyze data Guide students Interpret data Lab scale batches Technology research Use a confocal microscopy Write papersCollaborate with principal investigatorCollaborate with project teamConduct literature searchesConduct research at universitiesConfocal microscopyCreates a collaborative team environmentData analysisDesign scientific projects (in biology)Determine statistical analysis Execute scientific projectslifescienceManuscript reviewManuscript preparation and reviewOral presentationOrganise meetingsOrganize scientific projects (in biology)Solve problemsStatistical analysisStorytellingWrite papers
Doctor of Philosophy (Ph.D.) in Applied Microbiology from Lunds tekniska högskola / The Faculty of Engineering at Lund University in 2013
Master of Science (M.Sc.) in Biotechnology from Lunds tekniska högskola / The Faculty of Engineering at Lund University in 2006