- Versatile scientist with strong background in Chemistry, Biochemistry, Molecular Biology and Clinical Trials. - Extensive experience in the study of cellular signalling pathways involved in cancer, inflammation and immune diseases. - Efficient and fast learner, with a culture of continuous improvement and goal-oriented. - Creative problem solver, multi-tasking and adaptive to changing environments. - Self motivated as well as a great team player, and an effective writer and communicator. - Passionate about biomedical research, drug discovery and clinical trials.
Postdoctoral Scientist, Cell Signalling Brussels, Belgium
Since September 2013
Project management. Research focused in two main projects: one dedicated to study of a molecular mechanism involved in inflammation, linked to autoimmune diseases and tumorigenesis. And another project dedicated to characterize the function and regulation of a specific deubiquitinating enzyme during the cell cycle in cells under pathological conditions (brain cancers). Working with mammalian cell cultures. Transfection and transformation techniques. Protein purification. Molecular cloning, site-directed mutagenesis, DNA/RNA extraction, purification and quantification. Polymerase Chain Reaction (PCR). DNA gel electrophoresis. In vitro assays for protein phosphorylation and protein ubiquitylation. Immunoassays, radioisotopes, reticulocyte lysate, protein immunoprecipitacion and luciferase assay. Quantitative western blot analysis applied to the study of signalling pathways. Mentoring and training graduated students and technicians. Write and critically review reports, protocols and peer-reviewed publications. Design and coordinate laboratory activities towards a successful completion. Present results in front of the scientific community.
Research Scientist - Doctor in Chemistry (PhD in Protein Chemistry) Spain/Belgium
July 2009 --- September 2013
Title PhD Thesis: Versatility in Proteins Interactions Involving Modular Domains of Adaptor Proteins.
Working in multicultural teams, project management and publication of high impact peer-reviewed papers in international scientific journals. In depth data management and interpretation.
Hands-on laboratory experience in protein expression, cell culture (prokaryotic and eukaryotic cells), purification of recombinant proteins, separation techniques chromatography, isotopic labelling protocols and Immunoassays.
High experience in complex biophysical techniques applied to the study of protein stability, dynamic and protein interactions, such as: Isothermal Titration Calorimetry, Nuclear Magnetic Resonance spectroscopy, Circular Dichroism, Fluorescence spectroscopy, Small Angle X-ray Scattering and Bio-layer Interferometry.
Techniques used: - Nuclear Magnetic Resonance (NMR) espectroscopy - Isothermal Titration Calorimetry (ITC) - Circular Dichroism (CD) - Fluorimetry - Bio-layer interferometry - Titrations: acid-base, redox and complexometric - Small Angle X-ray Scattering (SAXS) - Liquid chromatography (HPLC and FPLC) especially for protein purification: ionic exchange, size exclusion (gel filtration), reverse phase and affinity chromatography - Sodium dodecyl sulfate-polyacrylamide gel electrophoresis. - Cloning and expression of recombinant proteins in prokaryotes. - Protein overexpression in different growth media, including 13C, 15N-enriched minimal media for isotopic labelling. - Purification of tagged (GST-, His-, Flag-… tags) and untagged proteins, including the design of new protein purification protocols. Cell lysis (e.g. sonication, French press and cell cracker), precipitation techniques (e.g. pH and ammonium sulphate precipitation), ultracentrifugation, purification in denaturing conditions (Urea, guanidinium), protein refolding (e.g. by dialysis, dilution and in-column refolding).
Research Scientist - Molecular Biology Spain
September 2008 --- July 2009
Research Collaboration - Biochemistry
January 2007 --- September 2008
Analytical thinkingResiliencyPerspectiveSelf-confidenceProblem solvingProactivityOrganizationFlexibilityEfficiencyCuriosityCoordinationAttention to detailSociability
Biochemistry Biostatistics Cell biology R&D Scientific writingAllergy and immunologyAntibodiesBioinformaticsBiological Drug DevelopmentBiomarkersBiopharmaceuticalsBiochemistryBiotechnologyCancerChemistryCell CultureClinical Data Management (CDM)Clinical monitoringClinical operationsClinical researchClinical Study DesignClinical study reportsClinical trial managementClinical trialsCMC – Chemistry activitiesData AnalysisData ManagementDNADNA extractionGCPICH GCP guidelinesImmunoprecipitationIn VitroInflammationInformed Consent ProcessLaboratoryMicrosoft ExcelMicrosoft OfficeMolecular & Cellular Biologymolecular biologyMolecular CloningMonitoring Study ProgressoncologyPCRPhases of clinical development (phase I to IV)PowerPointPrinciples and ethics of clinical researchProblem-solving methods and troubleshootingProject ManagementProtein ExpressionProtocolRT-PCRScientific WritingSDS-PAGEUnderstand levels of research evidenceUnderstanding of regulatory guidelinesWestern BlottingInformed Consent DocumentsWriting Study Procedures and SOPs
Skills and Expertise
Analyze data Create SOPs Develop clinical trial protocols Develop protocols Search literature on clinical trials Technology research Write papersAnalyze dataAnalyze proteinBiomarker Researchbudgeting of R&D activitiesChemistry Manufacturing and Controls (CMC) activitiesCoachCreate clinical documentsDesign protocolDesign study documentsEvaluate protocolsInterpret datalifesciencePublication of articlesProvide trainingReport dataResearch at universitiesReview data interpretationSolve problemsUse western blotting techniqueWrite papersWrite protocolsWritten presentation
Master in Clinical Trials in Clinical Trials from Universidad de Sevilla in 2014
PhD in Chemistry (Doctor in Chemistry) in Protein Chemistry-LifeSciences from University of Granada/Vrije Universiteit Brussels in 2013
Advance Master in Biochemistry and Molecular Biology in Structure and Function of Proteins from Universidad de Sevilla in 2010
Degree in Chemistry (SUMMA CUM LAUDE) in Chemistry from Universidad de Granada in 2008
Expert has 2 publications (Will be avalible with full profile)