January 2015 --- July 2016
My research focused on the elucidation of molecular mechanisms of cardiac glucose uptake inhibition by fatty acid. I seek the involvement of acetylation process in impaired GLUT4 translocation and diminished glucose uptake as a results of fatty acid oxidations in the heart. I established a technique to separate plasma membrane fractions from endosomal fractions with a series of ultracentrifugation steps and another technique to separate mitochondrial, cytosolic and nuclear fractions with a rather simple centrifugation steps. My research was awarded with two personal grants, however due to personal reasons I couldn’t pursue them. I have also contributed a book chapter to an edited volume on AMPK function in carbohydrate, lipid and protein metabolism.
April 2014 --- December 2014
During my work in the Molecular Physiology group of the NEXS department I participated in different projects related to glucose metabolism, became more familiar with skeletal muscle physiology and exercise and learned the techniques to study substrate and energy metabolism such as ex-vivo muscle incubations. Immunohistochemical staining of different cytoskeletal proteins in single fibres as well as transverse muscle sections and imaging constituted a big part of my work. My work in the NEXS department gave me two publications. Another manuscript (co-authored) which addresses beta-actin involvement in glucose utilization is under preparation for publication.
PhD in Cardiac Metabolism (Life Sciences)
April 2009 --- December 2013
My PhD research focused on the identification of molecular mechanism in the regulation of cardiac substrate uptake. My work revealed novel regulatory steps in fatty acid transporter CD36 and glucose transporter 4 (GLUT4) mediated cardiac fatty acid and glucose uptake in response to physiological stimuli like insulin and contraction.
AdaptabilityAnalytical thinkingAttention to detailAssertivenessCollaborationCommunicativeCritical thinkingCuriosityDiligenceIndependenceInterest in knowledgeKindnessOrganizationProblem solvingSelf-confidenceTrustWillingness to compromise
Biochemistry Diabetes Animal models Scientific writingBiomedical SciencesCardiologyCardiovascular diseasesCell CultureCell SignalingChromatographyConfocal MicroscopyDNA extractionElectrophysiologyELISAEndocrinology and metabolismHistologyImmunohistochemistry (IHC)ImmunoprecipitationInductively coupled plasma mass spectrometry (ICP-MS)In VivoInflammationLaboratory ResearchLC-MSLife SciencesMammalian Cell CultureMetabolismMolecular & Cellular BiologyNutritionPCRPhysiologyPresentation SkillsProteomicsqPCRRNA isolationRT-PCRScientific methodologyScientific WritingSDS-PAGEStatisticsTeamworkTransfectionUnderstand how results translate to practice
Skills and Expertise
Analytical skills Analyze data Develop protocols Guide students Interpret data Lab scale batches Report data Search literature on clinical trials Use a confocal microscopy Write papers Write protocolsAdjust methodsArchive study documentsassess the needs of the project(s)/program(s) and make changes in process, work flow and/or assignments.Assist with experimentsAttend at steering committee meetingsAttend seminarsCell cultureCollaborate with principal investigatorCollect dataConduct animal studiesConduct limited data and/or statistical analysis.Conduct close-out visitsConduct literature searchesConduct research at universitiesContact potential subjectsCoordinate with the ethics commiteeDesign protocolsDesign scientific experimentsDesign scientific projects (in biology)Execute scientific projectsInteract with ethics committeeInterpret research resultsInterpret scientific dataLiaise with research team in order to accurately collect and record dataLiaise with research teamlifescienceManuscript preparationManuscript reviewNetworkObtain grantsOral presentationPlan experimentsPrepare manuscriptPresent data at congressPublication of articlesRead medical literature to maintain current awareness and knowledgeResearch at universitiesReview dataReview study protocolsSolve problemsSupervise analysts and researchersSupervise PhD studentsSupervise techniciansUse fluorescence microscopyUse laboratory techniquesUse western blotting techniqueWrite papers
Master of Science in Medical Biochemistry from Dokuz Eylul University, the Health Science Institute in 2009
Bachelor of Science in Biochemistry from Ege Üniversitesi in 2006
Training and Certification
Advanced Microscopy and Vital Imaging Certification
Introduction to PBL (Problem Based Learning) and the role of the tutor Certification
Laboratory Animal Science, Article 9 Certification
Radiological Protection level 5b: Radiation safety license Certification
Expert has 9 publications (Will be avalible with full profile)