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Summary

my Research focus in the microbial & enzime technology Lab has been on the evolution and biochemical Characterization of new oxidoreductive bacterial enzymes (laccases, azo/quinone reductases, dyp-type peroxidases) to contribute to their improvement for biotechnological Applications as well as to better understand their mechanistic pathways. i have 14 Publications: 11 papers in peer-reviewed international journals (times cited: 92, h-index: 6), two as corresponding author, and 3 peer-reviewed conference proceedings, 7 oral and 14 poster communications at national and international conferences. i co-supervised 3 m.sc., 1 graduate, 4 undergraduate, 2 erasmus and 3 high-school students. i collaborated in fct “nanoengineering of bacterial laccases”, in eu biorenew (http://www.biorenew.org/) projects and in the exploratory fct project “Discovery and Characterization of ligninocellulolytic bacterial enzymes”. whilst developing my Research and fitting with my eagerness in learning i have become proficient in several techniques, such as molecular biology and genetic engineering (PCR, cloning techniques, Electrophoresis, Bacterial Transformation), robotic High-throughput screening (tecan and hamilton robots), Lab-scale Fermentation, Protein Purification, fast protein Liquid chromatography (fplc), UV-Vis and Fluorescence Spectroscopy, circular dichroism (cd), differential scanning calorimetry (dsc), high performance Liquid chromatography (HPLC) and stopped-flow Analysis.

Experiences

Current Experience

  • Postdoctoral Researcher


    Since December 2006
    i developed expression systems and various screenings for directed evolution. i used site-directed and saturation mutagenesis for structure-function studies and random mutagenesis and recombination approaches to increase the thermostability of azo/quinone reductase allowing simultaneously to identify the molecular basis of kinetic and thermodynamic stability. i studied the biochemical properties of two new dyp-type peroxidases with the potential to replace high-redox fungal lignin peroxidases in biotechnological Applications. I developed expression systems and various screenings for directed evolution. I used site-directed and saturation mutagenesis for structure-function studies and random mutagenesis and recombination approaches to increase the thermostability of azo/quinone reductase allowing simultaneously to identify the molecular basis of kinetic and thermodynamic stability. I studied the biochemical properties of two new DyP-type peroxidases with the potential to replace high-redox fungal lignin peroxidases in biotechnological applications.

Personality

Self Assessment :
AdaptabilityAnalytical thinkingAttention to detailAuthenticityCollaborationCritical thinkingIndependenceInterest in knowledgeResiliencyResponsibilityTrustWillingness to compromise

Knowledge

LinkedIn Assessment :
PCRProtein chemistrymolecular biologyGel ElectrophoresisBiochemistryCell CultureBiotechnologyProtein PurificationIn VitroProtein EngineeringHigh Throughput ScreeningProtein ExpressionProtein Characterization

Skills and Expertise

Self Assessment :
Analytical skills Analyze data Develop protocols Guide students Interpret data Lab scale batches Write papersAnalyze proteinAttend seminars, courses and meetings within and outside the companyCommunicationConduct literature searchesData validationData verificationEvaluate stability data Execute scientific projectsGuide studentsInterpret analytical resultsInterpret research resultsManuscript preparation and reviewOral presentationResearch at universities

Education

  • Doctor of Philosophy (Ph.D.) in Biotechnology from Instituto Superior Técnico in 2006
  • Visiting Research Scientist in from Institut für Molekulare Enzymtechnologie, Heinrich-Heine-Universität Düsseldorf, Germany in 2004
  • Chemical Engineering in Biotechnology from Instituto Superior Técnico in 2002

Languages

BrightOwl Assessment:
Self Assessment:
Portuguese
Native
English
Professional Proficiency
Spanish
Elementary Proficiency
German
Elementary Proficiency

Work Preferences

  • Locations I am interested in:
  • Work From Home:
    No
  • Work Regime:
    Permanent position
  • International:
    Yes

Area / Region

Lisbon, Portugal

Others

Driving License
  • Yes

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