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Summary

Involved in FP7 Venomics project 

http://www.venomics-project.eu

- de novo sequencing of pure peptidic toxins by mass spectrometry (top down and bottom up approaches)
- Development of a high-throughput de novo sequencing platform for peptidic toxins
- Bioinformatics (Peaks software, homemade software for de novo sequencing peptides, Matching Transcriptomics and Proteomics data)
- Quality control of toxins produced in E. coli


Mass spectrometer experience :

QExactive orbitrap (Thermo)
LTQ linear ion trap (Thermo)
MicroTOF (Bruker daltonics)
Ultraflex MALDI MS (Bruker daltonics)
SolariX FT-ICR (Bruker daltonics)
AmaZon ion trap (Bruker daltonics)
Synapt G1 (Waters)
Synapt G2 (Waters)

 

UPLC experience:

nanoAcquity (Waters)

I-class (Waters)

M-class (Waters)

Ultimate 3000 (Dionex-Thermo)

Experiences

Current Experience

  • PhD Student
    Since December 2011
    Involved in FP7 Venomics project http://www.venomics-project.eu - de novo sequencing of pure peptidic toxins by mass spectrometry (top down and bottom up approaches) - Development of a high-throughput de novo sequencing platform for peptidic toxins - Bioinformatics (Peaks software, homemade software for de novo sequencing peptides, Matching Transcriptomics and Proteomics data) - Quality control of toxins produced in E. coli Mass spectrometer experience : QExactive orbitrap (Thermo) LTQ linear ion trap (Thermo) MicroTOF (Bruker daltonics) Ultraflex MALDI MS (Bruker daltonics) SolariX FT-ICR (Bruker daltonics) AmaZon ion trap (Bruker daltonics) Synapt G1 (Waters) Synapt G2 (Waters)

Past Experience

  • Master Thesis Student
    September 2010 --- September 2011
    Characterization of proteins modified by drug reactive metabolites Metabolites used: acetaminophen/paracetamol, clozapin Protein model: beta lactoglobulin from milk (Bos taurus) - Identification of modification sites by mass spectrometry - Quantification of modification by C14 radiolabeled metabolites (to refine)

Knowledge

LinkedIn Assessment :
Mass SpectrometryAnalytical ChemistryProteomicsVenomicsAnalytical biochemistryDe novo sequencingBiochemistryBiochemical EngineeringColumn ChromatographyProtein PurificationProtein CharacterizationProtein ChromatographyAffinity ChromatographyHydrophobic Interaction ChromatographyToxinsData AnalysisMassLynxExcaliburPeaksSpectrométrie de masseBio-informatiqueBiochimieProtéomiquePurification des protéinesSciences de la vieCaractérisationBiologie moléculaireChimie analytique

Skills and Expertise

Self Assessment :
analytical chemistry Write papersWritten presentationResearch at universitiesQuality control processPublication of articles

Education

  • Master 2 in Biochimie et biologie moléculaire from Université de Liège in 2011
  • CESS in Sciences - Mathématiques from Athénée provincial de La Louvière in 2004

Languages

BrightOwl Assessment:
Self Assessment:
French
Native
English
Professional Proficiency
Dutch
Elementary Proficiency
Italian
Elementary Proficiency

Area / Region

Clavier, Belgique

Others

Driving License
  • Yes