i'm passionate about the science and technology. innovations in technology and development in Biomedical Sciences go hand in hand and my interest lies in working at the interface of technology and life science. during the course of my graduate in bio-Nanotechnology, i focussed at polishing my skills in Cell-engineering and Polymer synthesis for Biological application. for my master thesis, i focused on Validation of hiv nucleocapsid Proteins interaction with various human cellular Proteins. from my past experiences, i acquired vast knowledge in a range of vivid techniques that are directly applicable in my field of interest, ranging from Polymer material synthesis to genetic engineering and lithography procedures. my phd demanded for technological upgradation in digital microfluidic platform for solving Biological problems which are not possible to achieve with conventional procedures. my previous experience and skills helped me in understanding and delivering the optimum, as per the demands of my work. i have been successful in expanding my comfort zone at the interface of technology and Biology, and on the basis of my rich and vast experience, i'm keen on entering the competitive world of industry that meets my expectation, further improves my knowledge, and where i can significantly contribute in a positive way.
PhD entitled ' Digital microfluidics for single cell analysis'
Since April 2011
digital microfluidics (dmf) is a nascent technology in the field of microfluidics, where microliter sizes droplets can be manipulated on a 2-d surface, using electro-wetting-on-dielectric forces. however, the application of dmf technology in the field of single cells Analysis is left largely unexplored. in the course of my phd, i have worked on devising two Applications of dmf platform for conducting high throughput studies at single Cell resolution. the first work covered an application of dmf platform for conducting high throughput water permeability studies on magnetic bead bound arabidopsis thaliana protoplasts. conventional technique involved in conducting water permeability studies on protoplasts use labour intensive equipment like micropipettes and micromanipulator etc. the traditional method is time consuming, tedious and inefficient, as it allows Analysis of one protoplasts at a time. in the proof of concept demonstrated on dmf platform, an average of 7 protoplasts could be captured using magnetic actuations and monitored through a camera. in another work, i validated the application of dmf platform for capturing hundreds of single saccharomyces cerevisiae in a microwell array, and monitored the responses of single cells upon cytotoxic drug treatments for a period of 6 hours. it was for the first time, that a drug dose- Cell response relationship was elucidated on any platform at a high throughput. Digital microfluidics (DMF) is a nascent technology in the field of microfluidics, where microliter sizes droplets can be manipulated on a 2-D surface, using electro-wetting-on-dielectric forces. However, the application of DMF technology in the field of single cells analysis is left largely unexplored. In the course of my PhD, I have worked on devising two applications of DMF platform for conducting high throughput studies at single cell resolution. The first work covered an application of DMF platform for conducting high throughput water permeability studies on magnetic bead bound Arabidopsis thaliana protoplasts. Conventional technique involved in conducting water permeability studies on protoplasts use labour intensive equipment like micropipettes and micromanipulator etc. The traditional method is time consuming, tedious and inefficient, as it allows analysis of one protoplasts at a time. In the proof of concept demonstrated on DMF platform, an average of 7 protoplasts could be captured using magnetic actuations and monitored through a camera. In another work, I validated the application of DMF platform for capturing hundreds of single Saccharomyces cerevisiae in a microwell array, and monitored the responses of single cells upon cytotoxic drug treatments for a period of 6 hours. It was for the first time, that a drug dose- cell response relationship was elucidated on any platform at a high throughput.
Master Thesis entitled 'Interaction of HIV proteins with Human cells and their cellular partners'June 2010 --- January 2011
numerous drugs against hiv Virus have been developed since its Discovery, which after a prolonged use proved detrimental towards the good Health of patients because of unacceptable side-effects. the crucial challenge is to develop newer drugs with unfamiliar resistant strains of hiv, with maximum possible degree of endurance and benefits. researchers have already discovered that nucleocapsid Protein (ncp7) plays a very important role in viral functioning such as RNA Packaging, dimerization and maturation, RNA protection, reverse transcription and integration of proviral DNA. in that context, ncp7 is a novel target for antiretroviral Treatment.the objective of this study was to determine if ncp7 and gag can interact with rpl7 in a cellular context, with the aid of using different techniques (Microscopy and cellular and biochemical techniques).
InternMay 2009 --- August 2009
development of electrochemical biosensor for urea using conductometric route
Summer internship on 'chemical route to synthesize conducting polymer, Polyaniline'July 2008 --- August 2008
InternJuly 2007 --- August 2007
Analytical thinkingCommunicativeCompetitivenessCritical thinkingEfficiencyInterest in knowledgeProblem solvingResult Oriented
Skills and Expertise
Analytical skillsCleanroomUse fluorescence microscopy Analyze data Develop protocols Guide students Interpret data Technology research Write papers Write protocolsAttend seminarsCell culturecollaborate in research projects at universitiesCommunicationConduct literature searchesConduct university research Design scientific experimentsEnsure good clinical practice (GCP)Operate research equipmentOral presentationR&DStatistical analysisTeachingWork cross-functionallyGuide students Microfabrication
Doctor of Philosophy (Ph.D.) in Bioscience engineering from University of Leuven in 2016
Int. M.Tech in Nano-biotechnology from Amity Institute of Nanotechnology, Noida in 2010
Senior secondary in Maths Biotech from Subodh Public School in 2005
Secondary school in Science maths from Saint Anselm Pink City School in 2003
Training and Certification
Effective graphical display in 0000 Certification
Specialist course: Theory and methods of applied research in 0000 Certification
Expert has 2 publications (Will be avalible with full profile)