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Summary

PhD in Human Transmissible and Tropical Diseases, I am highly interested in the comprehension of the host-pathogen interactions in diseases and especially in tropical diseases. During my PhD, I worked for 5 years for the French Institute of Biomedical Research of the French Army (Marseille, France). I participated to the establishment of a clinical trial in Kinshasa (Democratic Republic of the Congo) and its follow-up, in association with medical team from the Monkole hospital. The objective of this trial was to better understand better the immunological components involved, according to the severity of malaria.

Since March 2014, for my post-doctoral fellowship, I study the development of trypanosomes during the early stages of the infection in the mammalian host by intravital imaging approaches in vivo (IVIS Spectrum and confocal microscope)using parasites expressing a fluorescent marker combine to a bioluminescent one and the implication of the flagellum in host-parasite interactions by the construction of strains expressing conditional knock-out. It allows me to develop new skills, especially in microscopy (confocal including Spinning Disk and CellVizio, epifluorescence), immunofluorescence and in vivo experimentation with mice (IP, IV or SC injection, blood sampling and dissection). 

Open-minded, curious and rigorous, I am highly involved in my work and in lab activities. My autonomy and capacities of organization are useful to lead head-on several projects. Competences: Imaging (confocal and epifluorescence microscopy, IVIS spectrum), biochemistry (Immunofluorescence, ELISA and multiplex technologies) molecular biology (PCR, real-time qPCR, whole blood extraction, sequencing, transcriptomic), cell, parasite and tsetse fly culture, animal manipulation in BSL-2 and BSL-3. I posses the animal testing diploma level I and a training for basics on laboratory rodent surgery.

Experiences

Current Experience

  • PhD researcher
    Since March 2014
    The purpose of my post-doc is to unravel the development of trypanosome parasites during the early stages of the infection in the mammalian host by intravital imaging approaches in vivo and the implication of the flagellum in host-parasite interactions. These parasites express a new triple reporter fusion protein combining the red-shifted firefly luciferase, developped in the lab, allowing to follow them at different scales ranging from whole organism to cellular level, both in vitro and in vivo in tsetse flies and murine models.

Past Experience

  • Doctorant en Pathologies humaine, spécialité maladies infectieuses, Paludisme
    September 2008 --- May 2013
    J’ai participé à l’élaboration et à la mise en place d’une étude clinique à Kinshasa (RDC, Afrique) visant à mieux comprendre les composantes immunologiques impliquées en fonction de la gravité de l'accès palustre présenté, en collaboration avec l’équipe médicale de l’hôpital Monkolé. Pour ce faire, j'ai dosé un large panel de médiateurs inflammatoires en méthode multiplex et amélioré le dosage en immunoessai classique de la réponse allergique de patients envers Plasmodium falciparum. J'ai complété cette étude de la réponse inflammatoire par une approche transcriptomique grâce à la technologie des puces à ADN. En parallèle, j'ai participé à la mise en place d’un criblage haut débit par technique d’immunoessai en multiplex. J’ai également contribué à l'amélioration du système de diagnostic d’espèce plasmodiale, au sein du laboratoire afin d’augmenter le débit et le rendement de celui-ci. J'ai également perfectionné ma technique en culture parasitaire et biologie moléculaire (PCR et PCR en temps réel). Au cours de cette formation, j’ai été amenée à collaborer à la gestion de divers budgets de fonctionnement et de projets scientifiques, et à élaborer des demandes de marchés publics. J'ai également pris la responsabilité du laboratoire de sécurité de niveau 3 de parasitologie, avec rédaction et mise à jour des procédures qualité.

  • Stage M2
    January 2008 --- September 2008
    Etude des mécanismes d’adaptation de P.falciparum à son environnement et caractérisation de sa mitochondrie.

  • Stage M1
    January 2007 --- July 2007
    Résistance de Plasmodium falciparum aux antipaludiques : surveillance de sa chimiosensibilité et recherche de nouvelles cibles parasitaires. Directeur de stage : Professeur Daniel PARZY. Compétences acquises : culture de Plasmodium falciparum, synchronisation de cultures parasitaires, évaluation in vitro de la chimiosensibilité de divers souches et isolats plasmodiaux retrouvés en Asie par comparaison de la méthode colorimétrique (ELISA) avec la méthode isotopique (hypoxanthine tritiéé).

  • Stage L3
    January 2006 --- February 2006
    Etude de l'activité antiproliférative d'un nouveau dérivé chlorambucile-homospermidine. Encadrant : Dr Delcros. Compétences acquises : culture cellulaire de cellules CHO et HELA, mesure de l'inhibition de croissance par cytométrie en flux, bonnes pratiques de laboratoire en laboratoire L2.

Personality

Self Assessment :
AdaptabilityAnalytical thinkingApproachabilityAuthenticityCollaborationCommunicativeCoordinationCuriosityEfficiencyDiligenceFlexibilityIndependenceKindnessOptimismOrganizationProblem solvingReaction to stressResiliencyResponsibilitySelf-disciplineSociabilityTrustWillingness to compromise

Knowledge

Self Assessment :
Animal models Biochemistry Cell biology Scientific writingAnalysisAnalytical techniquesAnalytical proceduresAnesthesiaAnimal HealthAnimal ModelsAnimal trialBacteriologyBioanalysisBiochemistryBiologyBiomarkersBiomedical SciencesBudgetsCell biologyCell Based AssaysCell CultureCell SignalingCleanroomClinical monitoringClinical researchClinical trialsCommunication SkillsComplianceConfocal MicroscopyCurrent Good Manufacturing Practice (CGMP)Data AnalysisData ScienceDNADNA extractionDNA sequencingELISAEnzyme AssaysFluorescence MicroscopyGel ElectrophoresisGood Laboratory Practice (GLP)ImmunoassaysImmunofluorescenceImmunologyIn VitroIn vitro diagnostics (IVD)In VivoInfectious diseasesInflammationLaboratoryLaboratory ResearchLaboratory SkillsLaboratory TechniquesLaboratory study designLife SciencesMammalian Cell CultureMicroscopyMicrosoft ExcelMicrosoft OfficeMicrosoft PowerpointMicrosoft WordMolecular & Cellular BiologyNucleic Acid ExtractionOutlookOxidative stressPatient Follow UpPatient Screening and RecruitmentPowerPointPresentationsProblem-solving methods and troubleshootingProteomicsProtocolqPCRreal-time PCRResearchSalesScientific WritingWestern Blotting
LinkedIn Assessment :
CellELISAPCRBiochemistryImmunologyLaboratoryreal-time PCRClinical trialsAnalysisMedical ResearchResearchmolecular biologyMicroscopie à fluorescenceMicroscope confocal

Skills and Expertise

Self Assessment :
Cell culturepcr Develop protocols Interact with physicians Interpret data Molecular Diagnostics Use a confocal microscopy Write protocols Write papersAdjust methodsAdminister logisticsAdjust processes and methodsAssist with experimentsAssist with proceduresAssist with routine testsAttend seminarsAttend seminars, courses and meetings within and outside the companyBiomarker ResearchCollaborate with medical teamCollaborate with principal investigatorCollaborate with project teamCollect patient forms and questionnairesConduct animal studiesConduct studiescontrol different protocol versions and other essential documentsControl studiesControl protocol versionsCoordinate projectsCoordinate logisticsDesign protocolsDesign scientific experimentsDesign studiesExecute scientific projectsEvaluate protocolsFollow up projectsGuide staffGuide studentsInteract with physiciansInteract with statisticiansInterpret dataInterpret analytical resultsInterpret research resultsInterpret scientific datalifescienceManage budgetsManage multiple projectsManage research projectsManuscript preparationManuscript preparation and reviewOptimize assay performanceOral presentationOrganize scientific projects (in biology)Plan experimentsPresent data at congressProcure and prepare samplesPublication of articlesReport technical problemsReview protocolsReview dataReview data interpretationSolve problemsSource animalsSupervise PhD studentsSupervise techniciansUnderstand protocolsUse fluorescence microscopyUse laboratory techniquesUse content management systemsUse laser doppling imaging techniqueUse western blotting techniqueVerify dataWrite documentsWrite papersWriting regulatory documentsWritten presentation

Education

  • Ph.D. in Human infectious diseases from Université Aix-Marseille in 2013
  • Master in Human infectious diseases from Aix-Marseille Université in 2008
  • Bachelor in Cell biology from Université Rennes I in 2006

Languages

BrightOwl Assessment:
Self Assessment:
French
Native
English
Professional Proficiency

Work Preferences

  • Positions I am interested in:
    Laboratory Engineer PhD researcher Postdoctoral Research Fellow Preclinical Scientist R&D engineer R&D Scientist Research Assistant researcher Scientific Researcher Biologist Clinical Research Physician Field Service Engineer
  • Locations I am interested in:
    Centre-Val de Loire, France Île-de-France, France
  • Work From Home:
    Yes, 0 to 5 days per week
  • Work Regime:
    Permanent position
  • International:
    No

Publications

    Expert has 7 publications (Will be avalible with full profile)

Area / Region

Paris, France

Others

Driving License
  • Yes