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Summary

a common response cells have to environmental cues and external stress is a change in redox state, causing internal enzymes to become modified by oxidation or reduction, and hence allowing the activity of these enzymes to be regulated. i am interested in a particular subset of enzymes, called Protein-tyrosine phosphatases (or ptps), which become inactivated when oxidized and thereby permit Signal Transduction of external stimuli, leading to cellular responses. the focus of my phd project is demonstrating ptp oxidation In Vivo in the zebrafish and discovering the role of ptp inactivation in the regeneration of the zebrafish caudal fin. to achieve this i make use of mutant knockout and transgenic zebrafish, fluorescent Confocal Microscopy and molecular techniques to study Protein dynamics and function.

Experiences

Current Experience

  • PhD student

    Utrecht, Netherlands
    Since November 2012

    a common response cells have to environmental cues and external stress is a change in redox state, causing internal enzymes to become modified by oxidation or reduction, and hence allowing the activity of these enzymes to be regulated. i am interested in a particular subset of enzymes, called Protein-tyrosine phosphatases (or ptps), which become inactivated when oxidized and thereby permit Signal Transduction of external stimuli, leading to cellular responses. the focus of my phd project is demonstrating ptp oxidation In Vivo in the zebrafish and discovering the role of ptp inactivation in the regeneration of the zebrafish caudal fin. to achieve this i make use of mutant knockout and transgenic zebrafish, fluorescent Confocal Microscopy and molecular techniques to study Protein dynamics and function.

    A common response cells have to environmental cues and external stress is a change in redox state, causing internal enzymes to become modified by oxidation or reduction, and hence allowing the activity of these enzymes to be regulated. I am interested in a particular subset of enzymes, called protein-tyrosine phosphatases (or PTPs), which become inactivated when oxidized and thereby permit signal transduction of external stimuli, leading to cellular responses. The focus of my PhD project is demonstrating PTP oxidation in vivo in the zebrafish and discovering the role of PTP inactivation in the regeneration of the zebrafish caudal fin. To achieve this I make use of mutant knockout and transgenic zebrafish, fluorescent confocal microscopy and molecular techniques to study protein dynamics and function.

  • PhD in Regenerative Medicine and Biochemistry


    Since November 2012
    investigating the role of Protein tyrosine phosphatases in the regeneration of the zebrafish fin. the earliest known response in the conserved process of epimorphic regeneration is a burst of hydrogen peroxide production at the site of injury (niethammer et al., 2009). although inhibition of either hydrogen peroxide production or many of the signalling pathways involved impairs zebrafish caudal fin regeneration (yoo et al., 2012, love et al., 2013), the mechanism that connects this hydrogen peroxide burst to the coordinated activation of multiple signalling pathways that ultimately drive regeneration is not known. in recent years hydrogen peroxide has emerged as an important second messenger Molecule in Cell biology. Protein-tyrosine phosphatases are a large family of regulators of Signal Transduction, reversing the phosphorylation of substrates, and have been shown to be essential in embryonic development (van der sar et al., 1999). Protein-tyrosine phosphatases are also highly sensitive to reversible oxidation-mediated inhibition by hydrogen peroxide (den hertog et al., 2005). my Research aims to identify the causal role between this hydrogen peroxide burst and oxidation-induced inhibition of Protein-tyrosine phosphatases in regeneration. Investigating the role of protein tyrosine phosphatases in the regeneration of the zebrafish fin. The earliest known response in the conserved process of epimorphic regeneration is a burst of hydrogen peroxide production at the site of injury (Niethammer et al., 2009). Although inhibition of either hydrogen peroxide production or many of the signalling pathways involved impairs zebrafish caudal fin regeneration (Yoo et al., 2012, Love et al., 2013), the mechanism that connects this hydrogen peroxide burst to the coordinated activation of multiple signalling pathways that ultimately drive regeneration is not known. In recent years hydrogen peroxide has emerged as an important second messenger molecule in cell biology. Protein-tyrosine phosphatases are a large family of regulators of signal transduction, reversing the phosphorylation of substrates, and have been shown to be essential in embryonic development (van der Sar et al., 1999). Protein-tyrosine phosphatases are also highly sensitive to reversible oxidation-mediated inhibition by hydrogen peroxide (den Hertog et al., 2005). My research aims to identify the causal role between this hydrogen peroxide burst and oxidation-induced inhibition of protein-tyrosine phosphatases in regeneration.

Past Experience

  • Peer assisted study scheme (PASS) leader

    October 2010 --- May 2011
    mentored a group of first year students; creatively constructing ways to help them learn lecture material, directing them to appropriate materials that will help promote their development. transferable skills: reliability; creativity; clear organisation of material; Flexibility; guiding the direction of discussion; explaining science at a level appropriate for the audience.

  • Classroom assistant

    July 2007 --- July 2009
    assisted teachers with administration and Teaching young kids the English language at an English language summer school for kids (2 weeks each year between the summer of 2007 and summer of 2009). skills: responsibility of care; patience; preparing and organizing material to a deadline.

  • Team Leader

    June 2006 --- September 2008
    albert heijn is the largest and highest-quality supermarket chain in the netherlands. i was employed at a medium-size branch (approximately 150 staff) for 6-12 hours per week. progressed from general shop-floor tasks to team Leader, with responsibility for opening the supermarket branch in the early morning and leading the pre-opening Operations team; most senior staff Member in the supermarket up to the time it opened to customers. skills: responsibility for producing results; team work; task Time Management; Teaching a process and the importance of that process to the whole business to junior team members; Leadership (incl. motivation, discipline and giving instructions to build an effective workforce).

Personality

Self Assessment :
Analytical thinkingAttention to detailCritical thinkingCreative thinkingCuriosityDiligenceFlexibilityInterest in knowledgeProblem solvingResponsibilityResult Oriented

Knowledge

LinkedIn Assessment :
ImmunologyProtein chemistryCell CultureqPCRscienceRing cloningForward and reverse transfection of siRNA and cDNATrypan blue stainingCrystal violet stainingCyQUANT cell proliferation assayTranscription factor luciferase assayTrizol RNA extractionFluorescent activated cell sorting (FACS) of cell cycle progressionExtraction and purification of mitochondriaSDS/Bis-Tris gel electrophoresisClick chemistry and in-gel fluorescenceCoomassie and silver staining of gelsImmunoblotting (ECL and Li-Cor)ImmunoprecipitationTrichloroacetic acid protein precipitationGlutathione S-transferase protein pull downRecombinant enterokinase cleavage capture of fusion proteinsReverse transcription PCRQualitative agarose gel PCRQuantitative real-time PCRNuclear Magnetic Resonance (NMR) spectroscopyGas chromatography-mass spectrometry (GC-MS)MicroinjectionmRNA synthesisLightsheet MicroscopyMini/Midi/Maxi-prepsChondrocyte differentiation/Phosphatase assaymolecular biologyCell biologyBiochemistryConfocal MicroscopyLife SciencesWestern BlottingMicroscopyResearchPolymerase Chain Reaction (PCR)

Education

  • Biomedical Research M.Res. in Oncology and Cancer Biology from Imperial College London in 2012
  • M.Res. in Biomedical Research from Imperial College London in 2012
  • B.Sc. in Biomedical Sciences from University of Manchester in 2011
  • Biomedical Sciences B.Sc. (Hons) in Biomedical Sciences, General from The University of Manchester in 2011
  • A levels in Biology, Chemistry, Maths, Dutch from British school in the Netherlands in 2008

Area / Region

Utrecht, Netherlands

Others

Driving License
  • Yes

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