BrightOwl Loader Loading

Summary

After several years expanding the depth and breadth of my scientific training as a pharmacologist in an academic setting, I look forward to applying my skills and experiences more directly to the development of novel therapeutics in the pharmaceutical industry. I am dedicated, adaptable, results-oriented, quality-minded, and I greatly enjoy collaborative/team-oriented environments. My strengths include: • Over 10 years experience in academic biomedical research ranging from basic molecular and cellular analysis to preclinical models of human disease • Preparing data/scientific material for manuscripts, presentations and grant/award applications • Extensive methods development and optimization • Project management and instruction/supervision of colleagues Specialties: Pharmacology, Signal Transduction, Molecular Biology, Biochemistry, Vascular Biology, Hematopoiesis, Thrombosis, Cell Biology, G-protein Coupled Receptors

Experiences

Current Experience

  • Postdoctoral Researcher
    Since September 2015
    Developing an effective transcriptional reporter assay as a downstream endpoint for GPCR activation/inhibition. Aiming to apply the assay to both a multiwell plate format as well as an "on-bead" assay, where cyclized peptide ligands, attached to a PEG-bead, can activate cells, bound to the beads, as a rapid means for screening libraries of peptide ligands.

Past Experience

  • Postdoctoral Researcher
    February 2014 --- August 2014
    As part of a short-term industrial partnership, I supervised colonies of genetic strains of mice with an aim toward evaluating the role of an orphan GPCR in CNS function. I also completed as part of a team a high throughput screen of several thousand small molecules against the same orphan GPCR in an attempt to identify new modulatory pharmacological scaffolds. Areas of Responsibility • Primary goals were to characterize the in vivo function of the orphan Gprotein coupled receptor GPR139 using mouse “gene knockout” models. Supervised the transfer of the mice to a KU facility after backcrossing to a standard genetic background. Harvested and sectioned brains for validation of knockout and autoradiographical evaluation of a high affinity ligand. • Additionally, completed a screen of a >4000 compound library against several GPCRs to identify possible positive and negative allosteric modulatory molecules using high throughput screening for cell signalling endpoints • Prepared final report for project

  • Associate Specialist
    October 2008 --- May 2012
    Have driven studies characterizing the role of G-proteins and GPCRs in the context of platelet function as it pertains to the processes of thrombosis and hemostasis in mouse models. Areas of Responsibility • Specifically, I examined the roles of G-protein coupled receptors and their associated signalling mechanisms in governing hematopoietic cell biology, with a specific emphasis on blood platelets. • Usage of preclinical animal models of disease was an important part of these studies. Results • Resulted in 9 publications in high impact journals. • Contributed to several collaborative studies. • Developed and implemented several methods; emphasis on platelet function and flow cytometry

  • Postdoctoral Researcher
    October 2002 --- October 2008
    Pursued several research aims utilizing murine and zebrafish model systems to explore the roles of the alpha subunits of Heterotrimeric G-proteins and their cognate G-protein coupled receptors in the context of innate immunity, hematopoiesis, and thrombosis.

Knowledge

LinkedIn Assessment :
pharmacologyBiochemistrymolecular biologyProtein ExpressionProtein PurificationCell CultureCell biologyCell Based AssaysCell SignalingAnimal Models of DiseaseThrombosisVascular BiologyFlow CytometryAnimal ModelsCellGeneticsSignal TransductionLaboratoryVascularClinical DevelopmentIn VivoFluorescence MicroscopyPhysiologyGPCRsConfocal MicroscopyscienceWestern BlottingTransfectionIn VitroMolecular CloningAssay developmentMicroscopyProject ManagementMethods OptimizationImmunofluorescenceTissue CultureLife Sciences

Education

  • PhD in Pharmacology and Toxicology from Queen's University in 2002
  • Bsc (Hons) in Life Sciences from Queen's University in 1996

Training and Certification

  • Fundamentals of Clinical Trials (Whitehall Training) Certification
  • ICH GCP in English (Whitehall Training) Certification
  • The Data Scientist’s Toolbox Certification

Languages

BrightOwl Assessment:
Self Assessment:
English
Native
Danish
Elementary Proficiency
French
Elementary Proficiency

Area / Region

Copenhagen, Denmark

Others

Driving License
  • No