Since September 2015
Developing an effective transcriptional reporter assay as a downstream endpoint for GPCR activation/inhibition. Aiming to apply the assay to both a multiwell plate format as well as an "on-bead" assay, where cyclized peptide ligands, attached to a PEG-bead, can activate cells, bound to the beads, as a rapid means for screening libraries of peptide ligands.
February 2014 --- August 2014
As part of a short-term industrial partnership, I supervised colonies of genetic strains of mice with an aim toward evaluating the role of an orphan GPCR in CNS function. I also completed as part of a team a high throughput screen of several thousand small molecules against the same orphan GPCR in an attempt to identify new modulatory pharmacological scaffolds. Areas of Responsibility • Primary goals were to characterize the in vivo function of the orphan Gprotein coupled receptor GPR139 using mouse “gene knockout” models. Supervised the transfer of the mice to a KU facility after backcrossing to a standard genetic background. Harvested and sectioned brains for validation of knockout and autoradiographical evaluation of a high affinity ligand. • Additionally, completed a screen of a >4000 compound library against several GPCRs to identify possible positive and negative allosteric modulatory molecules using high throughput screening for cell signalling endpoints • Prepared final report for project
October 2008 --- May 2012
Have driven studies characterizing the role of G-proteins and GPCRs in the context of platelet function as it pertains to the processes of thrombosis and hemostasis in mouse models. Areas of Responsibility • Specifically, I examined the roles of G-protein coupled receptors and their associated signalling mechanisms in governing hematopoietic cell biology, with a specific emphasis on blood platelets. • Usage of preclinical animal models of disease was an important part of these studies. Results • Resulted in 9 publications in high impact journals. • Contributed to several collaborative studies. • Developed and implemented several methods; emphasis on platelet function and flow cytometry
October 2002 --- October 2008
Pursued several research aims utilizing murine and zebrafish model systems to explore the roles of the alpha subunits of Heterotrimeric G-proteins and their cognate G-protein coupled receptors in the context of innate immunity, hematopoiesis, and thrombosis.
PhD in Pharmacology and Toxicology from Queen's University in 2002
Bsc (Hons) in Life Sciences from Queen's University in 1996
Training and Certification
Fundamentals of Clinical Trials (Whitehall Training) Certification
ICH GCP in English (Whitehall Training) Certification
The Data Scientist’s Toolbox Certification