BrightOwl Loader Loading

Experiences

Current Experience

  • Student
    Since August 2014
    > Specialization in Project Management and Finance > Researched and developed a marketing plan for a start up company. > Winner of Dow Industrial Challenge 2015.(Terneuzen, Netherlands) > Selected for 1.2.Startup event at YES!DELFT. Developed a business plan and pitched the business idea for a startup company "Lazarus Project".

Past Experience

  • Research Associate II
    January 2013 --- May 2014
    > Reporting to Research Scientist-I and member of six sigma green belt project. > Completed 7 research projects in 17 months.(4 as study director and 3 as team member) > Handling of blood plasma suspension for Factor VIII purification. > Centrifugation and filtration (Depth filtration) for Factor VIII purification. > Packing of Q-Sepharose and MAb affinity column. > Working with AKTA Explorer and Avant purification systems. > Method writing and editing in Unicorn 6.2. > Scale down and scale up calculations for column chromatography. > Column integrity testing and method development. > Albumin binding studies on Q-sepharose column. > Assisted on pre-column filter studies. > Performed ELISAs using spectramax. > DOE screening and optimization studies. > Using minitab for DOE setup and analysis. > Technology transfer to manufacturing team. > Co-coordinating sample testing and logistics with quality department. > Experiment planning,lab Management, SOP and protocol writing. > Trained in GDP and cGMP. > Provided training to personnel on working with human plasma suspension and ELISA.

  • Research Assistant
    August 2009 --- May 2012
    • Designed and constructed vectors for protein expression and purification. • Isolated and purified plasmid and genomic DNA. • Used various techniques like PCR, restriction digestion, ligation and transformation for molecular cloning. • Performed DNA gel electrophoresis. • Performed small scale fermentation operation for protein expression (2 L). • Expressed His tagged protein (Deformylase) in E.coli. • Optimized protein yields and conducted whole cell assays for protein activity. • Optimized protein purification (AKTA prime) using Immobilized metal affinity column chromatography. • In vitro assay development. • Kinetics and Substrate studies. • Used various techniques like HPLC, GC-MS-FID, UV-Vis Spectrophotometer and SDS-PAGE for protein characterization. • Collaboration with research groups for biophysical studies.

  • Engineer I
    August 2012 --- January 2012
    • Reporting to Senior Specialist, Fermentation. • Operated and supervised 15 liter fermentation tanks using Delta V control system. • Worked with SIP and CIP systems for 15 liter tanks. • Routine tank maintenance and troubleshooting. • Worked in BSL-2 environment (Haemophilus influenzae type b). • Used minitab for DOE setup and analysis. • Completed Lean Management Project (establishment of inventory system for BSL-2 lab). • Writing experimental summary and analysis. • Trained in GDP and cGMP.

Personality

Self Assessment :
Analytical thinkingAdaptabilityCommunicativeStrategic thinkingSelf-disciplineSelf-confidenceResult OrientedResponsibilityInterest in knowledgeInnovative thinkingFlexibility

Knowledge

LinkedIn Assessment :
Microbial FermentationProtein PurificationProtein Expressionmolecular biologyBioinformaticsFermentationProtein chemistryCellSDS-PAGEHPLCPurificationELISAMarketing Strategy

Education

  • Master's Degree in Management of Technology from Delft University of Technology in 2016
  • MS in Microbial Engineering from University of Minnesota-Twin Cities in 2012

Training and Certification

  • Six Sigma Green Belt Certification

Languages

BrightOwl Assessment:
Self Assessment:
English
Native
Dutch
Elementary Proficiency

Work Preferences

  • Locations I am interested in:
    Belgium Canada Denmark Germany Ireland Netherlands Singapore UK
  • Work From Home:
    No
  • Work Regime:
    Permanent position
  • International:
    Yes

Area / Region

Delft, Netherlands

Others

Driving License
  • No