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Experiences

Current Experience

  • Student


    Since August 2014
    > specialization in Project Management and Finance > researched and developed a Marketing plan for a start up company. > winner of dow industrial challenge 2015.(terneuzen, netherlands) > selected for 1.2.startup event at yes!delft. developed a business plan and pitched the business idea for a startup company "lazarus project". > Specialization in Project Management and Finance > Researched and developed a marketing plan for a start up company. > Winner of Dow Industrial Challenge 2015.(Terneuzen, Netherlands) > Selected for 1.2.Startup event at YES!DELFT. Developed a business plan and pitched the business idea for a startup company "Lazarus Project".

Past Experience

  • Research Associate II

    January 2013 --- May 2014
    > reporting to Research Scientist-i and Member of Six Sigma green belt project. > completed 7 Research projects in 17 months.(4 as study Director and 3 as team Member) > handling of blood plasma suspension for factor viii Purification. > centrifugation and filtration (depth filtration) for factor viii Purification. > packing of q-sepharose and mab affinity column. > working with akta explorer and avant Purification systems. > method writing and Editing in unicorn 6.2. > scale down and scale up calculations for column Chromatography. > column integrity Testing and method development. > albumin binding studies on q-sepharose column. > assisted on pre-column filter studies. > performed elisas using spectramax. > doe screening and optimization studies. > using minitab for doe setup and Analysis. > Technology transfer to Manufacturing team. > co-coordinating sample Testing and Logistics with quality department. > Experiment Planning,Lab management, SOP and science)" rel="nofollow">Protocol writing. > trained in gdp and cGMP. > provided Training to personnel on working with human plasma suspension and ELISA.

  • Research Assistant

    August 2009 --- May 2012
    • designed and constructed vectors for Protein Expression and purification. • isolated and purified plasmid and genomic DNA. • used various techniques like PCR, restriction digestion, ligation and transformation for molecular cloning. • performed DNA Gel Electrophoresis. • performed small scale Fermentation operation for Protein Expression (2 l). • expressed his tagged protein (deformylase) in e.coli. • optimized protein yields and conducted whole Cell Assays for protein activity. • optimized Protein Purification (akta prime) using immobilized metal affinity column Chromatography. • In Vitro Assay development. • kinetics and substrate studies. • used various techniques like HPLC, GC-MS-fid, UV-Vis spectrophotometer and SDS-PAGE for protein Characterization. • collaboration with Research groups for biophysical studies.

  • Engineer I

    August 2012 --- January 2012
    • reporting to senior specialist, Fermentation. • operated and supervised 15 liter Fermentation tanks using delta v control system. • worked with sip and cip systems for 15 liter tanks. • routine tank maintenance and Troubleshooting. • worked in bsl-2 environment (haemophilus influenzae type b). • used minitab for doe setup and Analysis. • completed lean management project (establishment of inventory system for bsl-2 Lab). • writing experimental summary and Analysis. • trained in gdp and cGMP.

Personality

Self Assessment :
Analytical thinkingAdaptabilityCommunicativeStrategic thinkingSelf-disciplineSelf-confidenceResult OrientedResponsibilityInterest in knowledgeInnovative thinkingFlexibility

Knowledge

LinkedIn Assessment :
Microbial FermentationProtein PurificationProtein Expressionmolecular biologyBioinformaticsFermentationProtein chemistryCellSDS-PAGEHPLCPurificationELISAMarketing Strategy

Education

  • Master's Degree in Management of Technology from Delft University of Technology in 2016
  • MS in Microbial Engineering from University of Minnesota-Twin Cities in 2012

Training and Certification

  • Six Sigma Green Belt in 0000 Certification

Languages

BrightOwl Assessment:
Self Assessment:
English
Native
Dutch
Elementary Proficiency

Work Preferences

  • Locations I am interested in:
    Belgium Canada Denmark Germany Ireland Netherlands Singapore UK
  • Work From Home:
    No
  • Work Regime:
    Permanent position
  • International:
    Yes

Area / Region

Delft, Netherlands

Others

Driving License
  • No

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