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Summary

i am an experienced Lab Technician(12 years). i have work experience in 

molecular biology:PCRSequencingDNA preparation: mini, maxi, giga, restriction digests, gel extraction, ligation,transformation, colony screening.

Cell biologyCell Culture, creation and maintenance of stable cell lines, Transfection,transduction, vector production

Biochemistry:Purification of Proteins by Immunoprecipitation, kinase test with Gel Electrophoresis and autoradiography,metabolic labelling, ELISA.

electrophoresis: DNA electrophoresis, SDS-PAGE, gel staining: coomassie and silver stain,Western Blotting.

recombinant Proteins:induction test of small and large cultures.first step affinity Purification.

mouse models and processing of human biopsies: handling mice, collection of blood and other tissue samples in mice, injection of viral vectors in mice

Histologycryostat and paraffin sectioning, histochemistry, Immunohistochemistry.

my contract was ended in may, 2015. at this moment i am urgently looking for a job.

 

 

Experiences

Past Experience

Personality

Self Assessment :
AdaptabilitySelf-confidenceProblem solvingIndependenceFlexibilityEfficiencyDependabilityCoordinationCollaborationAttention to detailTrust

Knowledge

Self Assessment :
Labtechnician Cell biology Animal models R&D BiochemistryBiological Drug DevelopmentCell CultureCardiovascular diseasesAntibodiesELISAEnglishFlow CytometryFluorescent microscopyImmunohistochemistry (IHC)MicroscopyMicrosoft ExcelMolecular & Cellular BiologyNeurosciencePCRToxicologyTransfection

Skills and Expertise

Self Assessment :
Develop protocolsAdjust methodsAdjust processes Assist with routine testsAssist with experimentsUse western blotting techniqueAlzheimer Technology researchAnalyze proteinlifescienceShipment of biological specimen

Education

  • Ph.D. in Biology(Morphology) from Catholic university of Leuven(KULeuven), Belgium in 1999
  • Master in Zoological Sciences from Catholic university of Leuven(KULeuven), Belgium in 1990
  • Bachelor in Environmental Biology from Jinan University, China in 1987

Training and Certification

  • Radioprotection in 2003 Certification
  • Animal Science(Proefdierkunde FELASA B) in 2002 Certification

Languages

BrightOwl Assessment:
Self Assessment:
English
Full Proficiency
Dutch
Professional Proficiency

Work Preferences

  • Notice Period:
    1 week
  • Positions I am interested in:
    Clinical Laboratory Technician Clinical Research Associate (CRA) Junior Laboratory Technician Lab Technician (Toxicology) Lab Technician Regulatory Affairs Assistant Research Assistant Research Scientist Veterinary Clinical Research Sr. Associate
  • Locations I am interested in:
    Belgium
  • Work From Home:
    No
  • Work Regime:
    Permanent position
    BrightOwl employee
  • International:
    Yes

Publications

    Expert has 6 publications (Will be avalible with full profile)

Area / Region

Herent, Belgium

Others

Driving License
  • Yes

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