Scientist Diepenbeek, Belgium
Since October 2016
Development of a porcine-derived medicinal cell product for type 1 diabetes patients.
Ph.D. Student Hasselt, Belgium
September 2012 --- September 2016
Exploring the Myocardial Regenerative Capacities of Cardiac Atrial Appendage Stem Cells for myocardial infarction patients.
Exploring and comparing the angiogenic properties of different dental stem cell populations
November 2011 --- June 2012
The angiogenic potential of 3 stem cell populations derived from human third molars was explored: dental pulp stem cells (DPSCs), dental follicle stem cells (DFSCs) and stem cells from the apical papilla (SCAPs), with the emphasis on their paracrine actions. A human gingival fibroblast cell line (HGF-1) was also included. The various DSC populations and the HGF-1 differentially express both pro- and anti-angiogenic factors, such as IGFBP-3, IL-8, TIMP-1 and VEGF, on mRNA and protein level. VEGF was even shown to be present in situ in all dental tissues. Functional in vitro assays showed induction of EC migration and tube formation, but not EC proliferation by all DSCs. No effects were found in the CAM assay. Based on marker expression, ultrastructure and suitability for therapeutic applications, an OG isolation method was preferred over an EZ technique. Secondly, it was shown that the human third molar may serve as a single source for 3 different stem cell populations with angiogenic potential. These results indicate that therapeutic angiogenesis with DSCs would provide opportunities to treat many patients, simply with cells gathered from their own or other individuals’ teeth.
Human umbilical cord matrix-derived stem cells as potential therapy for Multiple Sclerosis.
January 2011 --- April 2011
Umbilical cord Wharton’s Jelly-derived mesenchymal stem cells (UCMSCs) are proposed to be immune privileged because of their fetal origin. Therefore, their potential in multiple sclerosis treatment was investigated in this study. The umbilical cord serves as an easy accessible source for stem cells isolation without ethical issues, making UCMSCs ideal for clinical use. Human and rat umbilical cord mesenchymal stem cells were isolated by means of an explant technique without enzymatic treatment. The stem cells could be easily expanded and showed clonogenic capacities in CFU-F assays. However, human cells derived from different cord donors did show heterogeneous growth kinetics. The quality of both hUCMSCs and rUCMSCs was guaranteed by demonstration of mesenchymal stem cells marker expression and adipocyte differentiation potential. The surface marker expression profile of the hUCMSCs indicated a low immunogenicity and no alloresponse was elicited after coculture experiments with peripheral blood mononuclear cells. These latter findings make the use of hUCMSCs in multiple sclerosis and other immune mediated diseases promising.
Role of the glycine receptor in the proliferation and migration of cortical neurons in the embryo.
April 2010 --- June 2010
Neurotransmitters are not only important for signaling processes in the CNS, but also play a role in brain development and neurological disorders. Because functional glycine receptors are expressed on neuronal progenitors even before the formation of synapses, a role in development is suggested. Therefore, the effect of the glycine receptor on the proliferation and migration of cortical neurons was investigated. Embryonic mice brains were isolated at E18 and put in ex vivo slice cultures. Neuronal stem cells were labeled with BrdU and glycine receptors were blocked with strychnine. The effect on proliferation and migration was subsequently investigated by immunohistochemistry. A uniform rise in proliferation was found, insinuating that the glycine receptor inhibits the proliferation of cortical stem cells. On the other hand, glycine receptor signaling seems to stimulate migration, but this effect was less consistent. Nevertheless, a role for the glycine receptor in cortical development was clearly shown.
Result OrientedAttention to detailCollaborationProblem solvingProactivityApproachabilityCommunicativeAnalytical thinkingCoordinationIndependenceInterest in knowledgeKindnessSelf-disciplineFlexibilityCuriosityOptimismTrust
large animal models Cell biology Animal models Biostatistics Scientific writingAngiogenesisAnimal HealthAssay developmentBiomedical SciencesCardiologyCell Based AssaysCell CultureCell SignalingcGMPCharacterizationClinical researchCoachingCommunication SkillsCurrent Good Manufacturing Practice (CGMP)DNA extractionDNAELISAEnglishEthics submission and approval processEvent ManagementFACSFlow CytometryFluorescence MicroscopyGood Clinical Practice (GCP)Good Laboratory Practice (GLP)Grant PreparationGrant WritingGraphPad PrismHealthcareHistologyHospitalsImmunocytochemistry (ICC)ImmunofluorescenceImmunohistochemistryIn VitroInformed Consent DocumentsLaboratoryLaboratory SkillsLaboratory ResearchMarketingMedical ImagingMicrosoft Officemolecular biologyMolecular & Cellular BiologyNMRPCRpeople managementPhenotype Pre-clinical researchPreclinical developmentPreclinical regulations and practicesPreclinical SafetyPresentation SkillsProblem SolvingProject CoordinationProject ManagementProject PlanningProtein ExpressionProtocolPublic SpeakingqPCRQualitative ResearchQA complianceQuality Control (QC)Quality Management System (QMS)Quality SystemsQuantitative PCR (qPCR)Report WritingRNA isolationScientific WritingSPSSStandard Operating Procedure (SOP)Statistical Data AnalysisStem cell researchTeachingTeamworkTime ManagementTissue CultureTranslational MedicineWestern BlottingWriting Study Procedures and SOPs
Skills and Expertise
Cell cultureELISAFlow cytometryflow sortinghypoxia assaysimmunofluorescencein vitro modelslentivirus productionmicroscopyquantitative polymerase chain reactionworking with small animal models (mice and rats)working with large animal models (minipigs: MRINoga mappingAnesthesiabasic surgery techniques)peripheral blood mononuclear cell isolation and immunomodulation assaysStatistical analysiswestern blotCell culture ELISA Flow cytometry flow sorting hypoxia assays Immunofluorescence in vitro models lentivirus production microscopy quantitative polymerase chain reaction working with small animal models (mice and rats) working with large animal models (minipigs: MRI Noga mapping anesthesia basic surgery techniques) peripheral blood mononuclear cell isolation and immunomodulation assays statistical analysis Western Blot
in Life Sciences from Universiteit Hasselt in 2012
Master in Clinical and Molecular Sciences from University Hasselt in 2012
Bachelor in Biomedical Sciences from University Hasselt in 2010
Training and Certification
Clean room basics in 2016 Certification
Good clinical practice in 2016 Certification
Modules Marketing & Strategy, Business HR, Marketing in 2016 Certification
Expert has 2 publications (Will be avalible with full profile)