Since September 2011
The research work involved detection of chloroplast water in intact photosynthetic microorganisms such as Algae, Cyanobacteria and leaf disks. The theme of my Doctoral work was to study photosynthetic membrane (thylakoid membrane) dynamics in response to diverse environmental conditions using diffusion behaviour of chloroplast water, thylakoid membrane lipids and proteins. I developed non-invasive 1H NMR methods (DOSY and DRCOSY) to detect the chloroplast water in aforesaid intact systems. Using hindered diffusion of the chloroplast water, I estimated the size of chloroplast in leaf disks and also in algae. I also studied Chloroplast Volume Regulation in algae grown in different conditions using the chloroplast water diffusion. Most significantly, I could measure dynamics of the photosynthetic membrane lipids and proteins in vivo in algae suspension using strong magnetic field gradients. These were the first intact measurements on the mobility of lipids and proteins under complete in vivo conditions. Using these measurements I could probe changes in the membrane organization and composition.
September 2011 --- August 2015
Planning and designing experiments to detect and identify chloroplast water, lipids and proteins in leaf disks and in photosynthetic microorganisms such algae and cyanobacteria in order to study photosynthetic membrane dynamics.
Lecturer in Physics
June 2006 --- August 2008
Teaching: Mechanics, Optics, Thermodynamics, Nuclear Physics, Mathematical Physics and PC architecture and hardware.
Doctor of Philosophy (Ph.D.) in Biophysics from Wageningen University in 2016
Master's degree in Physiscs from University of Stuttgart in 2010
Master's degree in Physics from Osmania University in 2006