Perfectly trilingual (French, Dutch & English) Bioengineer, with first experience in protein chemistry R&D. Sociable, looking to expand knowledge and experience in the Biotech or Pharma industry in Belgium or abroad.
Master Thesis Brussels, Belgium
September 2013 --- September 2014
Master thesis in a research group focused on Urinary Tract Infections (UTI’s) and their causative agents (e.g. UPEC).
This thesis explored the potential development of a microbial adhesin as a tool for the investigation of pathogenesis of uropathogenic bacteria and as a novel diagnostic tool for specific types of inflamed tissues (e.g. tumors). During my Master Thesis I was required to generate mutants of the wild-type adhesin and identify their antigen-binding properties by developing, optimizing and validating an ELISA test followed by quantification of their affinity using a Biacore T200.
- Selection of to be altered (experimental set-up)
- Generation of adhesin mutants (genetic engineering and fermentation technology)
- Downstream processing - centrifugation, extraction, filtration, chromatograph
- Development and validation of an ELISA test for Adhesin-Antigen binding
- Quantification of binding properties of mutants using Biacore T200
Protein mutagenesis, production, purification and binding characterisation. Development, optimization and validation of ELISA.
March 2013 --- February 2014
Tutoring students in the fields of mathematics and science.
Summer Intern / Industrial Placement Cambridge, United Kingdom
July 2013 --- August 2013
Summer Intern, Biopharm R&D, GlaxoSmithKline, Cambridge, UK
Reference - Adam Walker (PhD), Adam.Z.Walker@gsk.com
Summer intern at a GSK department specialized in antibody-technology
During my two-month stay at Biopharm R&D I was expected to produce and purify one of their antibody-based flagship molecules, followed by a quality assessment of its biochemical properties, a biocomparability study comparing fresh vs identical but older material. Additionally I was required to investigate if it was possible to optimize the Production to Downstream Processing transition phase.
- Production of antibody (using fermentation technology)
- Protein Purification (downstream processing)
- Investigate biochemical properties (protein chemistry)
- Investigate and streamline Downstream Processing
- Attend daily staff meetings and presentations
Recombinant protein production, downstream processing and protein chemistry – characterization and quality control.
Analytical thinkingAdaptabilityCommunicativeCritical thinkingCollaborationIndependenceInterest in knowledgeKindnessOptimismOrganizationResiliencyResponsibilitySelf-confidenceSociabilityTrust
Biochemistry Cell biology R&D Scientific writing Lab scale batches LabtechnicianAnalytical techniquesAntibodiesBiochemistryBiological Drug DevelopmentBiopharmaceuticalsBiotechnologyCell CultureDiagnosticsDNADNA extractionDNA sequencingELISAEnglishEnzyme-linked immunosorbent assay (ELISA)GeneticsGood Laboratory Practice (GLP)ImmunologyIn VitroInfectious diseasesKnowledge of the drug development processLaboratory ResearchLaTeXLabtechnician BiostatisticsLaboratoryLaboratory TechniquesMicrobiological TechniquesMicrobiologyMicrosoft OfficeMolecular & Cellular Biologymolecular biologyMolecular CloningOxidative stressImmunofluorescencePatentsPathologyPCRPharmaceutical IndustryPilot scale batchesProduct developmentProtein ExpressionProtocolQuantitative PCR (qPCR)R&DRT-PCRScientific WritingSDS-PAGEStatistical considerations in design and analysisStatisticsTransfectionWestern Blotting
Master's degree in Bioengineering Sciences: Cell and Gene Biotechnology (Minor Medical Biotechnology) from Vrije Universiteit Brussel in 2014
Bachelor's degree in Bioengineering Sciences: Cell and Gene Biotechnology from Vrije Universiteit Brussel in 2012
ASO in Math/Sciences from Tachkemoni Antwerpen in 2008