Perfectly trilingual (French, Dutch & English) Bioengineer, with first experience in protein chemistry R&D.
Expected to complete my PhD in Chemical Engineering, at the end of 2020, at the departments of Chemical Engineering at the VUB and at Mesoscale Chemical Systems at the University of Twente.
Since December 2016
Master Thesis Brussels, Belgium
September 2013 --- September 2014
Master thesis in a research group focused on Urinary Tract Infections (UTI’s) and their causative agents (e.g. UPEC).
This thesis explored the potential development of a microbial adhesin as a tool for the investigation of pathogenesis of uropathogenic bacteria and as a novel diagnostic tool for specific types of inflamed tissues (e.g. tumors). During my Master Thesis I was required to generate mutants of the wild-type adhesin and identify their antigen-binding properties by developing, optimizing and validating an ELISA test followed by quantification of their affinity using a Biacore T200.
- Selection of to be altered (experimental set-up)
- Generation of adhesin mutants (genetic engineering and fermentation technology)
- Downstream processing - centrifugation, extraction, filtration, chromatograph
- Development and validation of an ELISA test for Adhesin-Antigen binding
- Quantification of binding properties of mutants using Biacore T200
Protein mutagenesis, production, purification and binding characterisation. Development, optimization and validation of ELISA.
March 2013 --- February 2014
Tutoring students in the fields of mathematics and science.
Summer Intern / Industrial Placement Cambridge, United Kingdom
July 2013 --- August 2013
Summer Intern, Biopharm R&D, GlaxoSmithKline, Cambridge, UK
Reference - Adam Walker (PhD), Adam.Z.Walker@gsk.com
Summer intern at a GSK department specialized in antibody-technology
During my two-month stay at Biopharm R&D I was expected to produce and purify one of their antibody-based flagship molecules, followed by a quality assessment of its biochemical properties, a biocomparability study comparing fresh vs identical but older material. Additionally I was required to investigate if it was possible to optimize the Production to Downstream Processing transition phase.
- Production of antibody (using fermentation technology)
- Protein Purification (downstream processing)
- Investigate biochemical properties (protein chemistry)
- Investigate and streamline Downstream Processing
- Attend daily staff meetings and presentations
Recombinant protein production, downstream processing and protein chemistry – characterization and quality control.
Analytical thinkingAdaptabilityCommunicativeCritical thinkingCollaborationIndependenceInterest in knowledgeKindnessOptimismOrganizationResiliencyResponsibilitySelf-confidenceSociabilityTrust
Biochemistry Cell biology R&D Scientific writing Lab scale batches LabtechnicianAnalytical techniquesAntibodiesBiochemistryBiological Drug DevelopmentBiopharmaceuticalsBiotechnologyCell CultureDiagnosticsDNADNA extractionDNA sequencingELISAEnglishEnzyme-linked immunosorbent assay (ELISA)GeneticsGood Laboratory Practice (GLP)ImmunologyIn VitroInfectious diseasesKnowledge of the drug development processLaboratory ResearchLaTeXLabtechnician BiostatisticsLaboratoryLaboratory TechniquesMicrobiological TechniquesMicrobiologyMicrosoft OfficeMolecular & Cellular Biologymolecular biologyMolecular CloningOxidative stressImmunofluorescencePatentsPathologyPCRPharmaceutical IndustryPilot scale batchesProduct developmentProtein ExpressionProtocolQuantitative PCR (qPCR)R&DRT-PCRScientific WritingSDS-PAGEStatistical considerations in design and analysisStatisticsTransfectionWestern Blotting
Master's degree in Bioengineering Sciences: Cell and Gene Biotechnology (Minor Medical Biotechnology) from Vrije Universiteit Brussel in 2014
Bachelor's degree in Bioengineering Sciences: Cell and Gene Biotechnology from Vrije Universiteit Brussel in 2012
ASO in Math/Sciences from Tachkemoni Antwerpen in 2008