I'm an expert research lab technician - head operator VRC FACS sorting core, working in the VRC-Lab of Prof. Peter Carmeliet since 2000.
Expert Laboratory Technician - Head operator VRC FACS sorting core, Vesalius Research Center, KUL Leuven, Belgium
Since January 2000
The Vesalius Research Center (VRC) has focused since 15 years its research interests on unraveling the molecular basis of neuro-vascular biological processes and diseases, and translating these genetic insights in therapeutic concepts – with the ultimate aspiration to develop novel treatments.
Being head operator, since July 2014 of our Flow Cytometry Core Facility, I sort and analyze samples researchers provide based on the criteria they have set.
A key component of this is providing advise on how they can improve their sample preparation for sorting to realize better results.
Maintaining and troubleshooting this high-tech sorter is essential to provide accurate and reproducible results and is something in which I take great pride.
The ultimate goal is providing the best possible service to my colleagues so they may proceed with their experiments.
> 13 years of experience in the histology field in an in-house histology core, which has developed into a sophisticated state-of-the-art histology and imaging facility. Our facility is equipped with the newest devices to perform automatic tissue embedding as well as paraffin, cryo and vibratome sectioning.
Expert in tissue preparation for histological analysis:
Embedding - sectioning (paraffin, cryo and vibratome)
Antibody stainings on cells, paraffin-, cryo and vibratome sections
Whole mount stainings
In situ hybridyzation
- Animal models -
FACS-based isolation of endothelial cells from various organs of mice
Perfusion and fixation of the vasculature through the heart
Tail vein injections
- Cell Culture -
EC isolation (Different organs of mice)
EC isolation (Human colon and Tumor)
Contact and meetings with representatives
Weekly meetings with Postdoc and MD PhD-students
Compose Risk assessments
Activity Promotor ActiefUnief
Setting up sports activities
FACSAria III sorting (Becton Dickinson) July 2014
FELASA B (Laboratory Animal Science) June 2014
HSE (Health - Safety - Environment) February 2012
Fire safety (First intervention team) May 2011
Initiatie draagbare blustoestellen (A-first asbl) March 2015
Course Account Management – Centrum Voor Avondonderwijs, Hasselt
Start date: September 2015
EfficiencyFlexibilityCreative thinkingProblem solvingCollaborationOptimismKindnessCuriositySelf-disciplineApproachabilityTrust
HistologyImmunohistochemistry (IHC)Flow Cytometry Animal modelsAntibodiesWriting Study Procedures and SOPsSOPIn VivoIn VitroCell CultureGood Laboratory Practice (GLP)Fluorescence MicroscopyCardiovascular diseasesCancerBiochemistry Medical devices Labtechnician Biochemistrymolecular biology
Skills and Expertise
Collaborate with medical teamCollaborate with principal investigatorCollaborate with project teamConduct research at universitiesSupervise techniciansSetup of research equipment
in from Regaschool Leuven in 0
in Mathematics - Languages from St-Jozefscollege Beringen in 0
Training and Certification
FacsAriaIII sorting in 2014 Certification
FELASA B Laboaratory Animal Science in 2014 Certification
EIP ( Eerste Interventieploeg ) in 2013 Certification
VGM - HSE in 2012 Certification