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I'm an expert research lab technician - head operator VRC FACS sorting core, working in the VRC-Lab of Prof. Peter Carmeliet since 2000.






Current Experience

  • Expert Laboratory Technician - Head operator VRC FACS sorting core, Vesalius Research Center, KUL Leuven, Belgium
    Since January 2000

    The Vesalius Research Center (VRC) has focused since 15 years its research interests on unraveling the molecular basis of neuro-vascular biological processes and diseases, and translating these genetic insights in therapeutic concepts – with the ultimate aspiration to develop novel treatments.

    Being head operator, since July 2014 of our Flow Cytometry Core Facility, I sort and analyze samples researchers provide based on the criteria they have set.
    A key component of this is providing advise on how they can improve their sample preparation for sorting to realize better results.
    Maintaining and troubleshooting this high-tech sorter is essential to provide accurate and reproducible results and is something in which I take great pride.
    The ultimate goal is providing the best possible service to my colleagues so they may proceed with their experiments. 


    > 13 years of experience in the histology field in an in-house histology core, which has developed into a sophisticated state-of-the-art histology and imaging facility. Our facility is equipped with the newest devices to perform automatic tissue embedding as well as paraffin, cryo and vibratome sectioning.

    Expert in tissue preparation for histological analysis:

    Embedding - sectioning (paraffin, cryo and vibratome)

    Antibody stainings on cells, paraffin-, cryo and vibratome sections


    Whole mount stainings

    In situ hybridyzation


    Other skills

     - Animal models -

    FACS-based isolation of endothelial cells from various organs of mice

    Perfusion and fixation of the vasculature through the heart

    Myocardial infarction

    Retro-orbital bleedings

    Tail vein injections



    - Cell Culture -

    EC isolation (Different organs of mice)

    EC isolation (Human colon and Tumor)

    HUVEC's (Umbilicals)

    BOEC (Blood)


    Additional responsibilities:

    Contact and meetings with representatives

    Weekly meetings with Postdoc and MD PhD-students

    Product management

    Waste Management

    Compose Risk assessments

    Compose SOP's

    Activity Promotor ActiefUnief

    Setting up sports activities 


    Additional certifications:

    FACSAria III sorting (Becton Dickinson) July 2014

    FELASA B (Laboratory Animal Science) June 2014

    HSE (Health - Safety - Environment) February 2012

    Fire safety (First intervention team) May 2011

    Initiatie draagbare blustoestellen (A-first asbl) March 2015


    Additional education:

    Course Account Management – Centrum Voor Avondonderwijs, Hasselt

    Start date: September 2015





Self Assessment :
EfficiencyFlexibilityCreative thinkingProblem solvingCollaborationOptimismKindnessCuriositySelf-disciplineApproachabilityTrust


Self Assessment :
HistologyImmunohistochemistry (IHC)Flow Cytometry Animal modelsAntibodiesWriting Study Procedures and SOPsSOPIn VivoIn VitroCell CultureGood Laboratory Practice (GLP)Fluorescence MicroscopyCardiovascular diseasesCancerBiochemistry Medical devices Labtechnician Biochemistrymolecular biology
LinkedIn Assessment :
HistologyAntibodiesAnimal ModelsImmunohistochemistry (IHC)HSE - VGMFACS SortingCryosectioningLaboratory SafetyWaste ManagementBiochemistryRisk AnalysisFire SafetyConfocal MicroscopyMicroscopyFACSFluorescence MicroscopyImmunologyAnalytical ChemistryEIPHuman Anatomymolecular biologySOP'sGel ElectrophoresisWestern BlottingGood Laboratory Practice (GLP)Good Distribution Practice (GDP)Cell CultureLaboratoryBiotechnologyscienceFlow CytometryResearch

Skills and Expertise

Self Assessment :
Collaborate with medical teamCollaborate with principal investigatorCollaborate with project teamConduct research at universitiesSupervise techniciansSetup of research equipment


  • in from Regaschool Leuven in 0
  • in Mathematics - Languages from St-Jozefscollege Beringen in 0

Training and Certification

  • FacsAriaIII sorting in 2014 Certification
  • FELASA B Laboaratory Animal Science in 2014 Certification
  • EIP ( Eerste Interventieploeg ) in 2013 Certification
  • VGM - HSE in 2012 Certification


BrightOwl Assessment:
Self Assessment:
Elementary Proficiency
Elementary Proficiency
Professional Proficiency

Work Preferences

  • Notice Period:
    12 weeks
  • Work From Home:
    Yes, 0 to 1 days per week
  • Work Regime:
    Permanent position
    BrightOwl employee
  • International:

Area / Region

Averbode, Belgium


Driving License
  • Yes