A Molecular Biologist with over 10 years of research experience and specialization in pathway engineering and protein expression platforms using E. coli, yeast and Lemna for the production of pharmaceuticals and nutraceuticals, including project management and R&D supervision. With a PhD in Molecular biology and a strong research background in industry as well as academic research institutes coupled with organizational and managerial skills including administration, scientific management and business development, I believe I would be able to do justice to the role.
Scientist - Assistant Director Bangalore, Karnataka, India
February 2014 --- March 2015
Manage and supervise R&D activities, Journals and Service platforms; Preparing project proposals for Grants; Project budgeting & management; Multi center project collaboration; Manage technical & non-technical staffs; Organizing CME, workshops & training programs.
Projects managed at ChanRe Rheumatology & Immunology Center & Research
(1) Simple method for early malaria diagnosis. (2) Comorbidities in Rheumatoid arthritis. (3) Microvascular complications in diabetes (T2D). (4) Study of NLR (neutrophil lymphocyte ratio) in Rheumatoid arthritis. (5) Study of EPC (endothelial progenitor cells) in diabetes.
Junior Research Fellow Vellore, Tamil Nadu, India
August 2008 --- May 2013
Established Lemna facility. Worked on expression and purification of engineered single chain variable fragment (scFv) antibody against TNFa using E. coli (periplasmic secretion) and Lemna expression systems.
Senior Research Fellow Bangalore, Karnataka, India
November 2005 --- July 2008
Pathway engineering in Yeast: Production of recombinant DHA.
Part of a team that established GCMS facility.
DOE using GCMS for FAME analysis.
Use of enhanceomers in vector designing for enhanced gene expression.
Projects completed at Avesthagen Limited, pharmaceutical / biotechnology company
Construction and verification of transfection ready recombinant expression constructs that separately encode the light and heavy chains of the recombinant monoclonal antibody- Bevacizumab (for CIPLA) and other in-house projects on sub-cloning of NESP; Cituximab monoclonal antibody; cloning of Tenecteplase; Glut4 and Xigris; Cloning of SAR/MAR using NESP and huIFNβSAR under Biotherapeutics division. In the Microbial process Development group - Bioneutraceuticals, I have worked on DHA pathway engineering in Yeast and fatty acid analysis using GCMS.
While working at Avesthagen Limited, I have contributed significantly for the patent:
Recombinant production of DHA (Docosahexaenoic acid) in Yeast: WO/2006/064317 (Pub. date: 22.06.2006)
Research Scholar Manipal, Karnataka, India
February 2005 --- July 2005
Worked on Pharmacogenomics of asthma, cardiovascular and psychiatric diseases.
Research Trainee Bangalore, Karnataka, India
May 2002 --- March 2003
Worked in the lab of Dr. P. Ajit Kumar, Department of Microbiology and Cell Biology. Got trained on biochemical and molecular biology techniques in the project: Biochemical characterization of ftsH and ftsZ proteins to study mechanism of cell division in M. tuberculosis
AdaptabilityInterest in knowledgeKindnessOptimismOrganizationOrientationProactivityProblem solvingResiliencySelf-confidenceSelf-disciplineService orientedSociabilityStrategic thinkingStrivingTrustInnovative thinkingIndividualityIndependenceAnalytical thinkingApproachabilityAssertivenessAttention to detailAuthenticityCollaborationCompetitivenessCoordinationCreative thinkingCritical thinkingCuriosityCharmDependabilityDiligenceEfficiencyWillingness to compromise
Antibodies Scientific writing R&D NegotiationDNA extractionELISAMicrobiologyLaboratoryInformed Consent ProcessIn VitroGeneticsGenetic polymorphismFluorescent microscopyEnglishWestern BlottingProof of conceptAssay developmentTherapeuticsDesign of ExperientAntibody engineering DiabetesProject ManagementProcess improvementPowerPointPharmacogenomics (study of genetic variation)Pharmaceutical IndustryPCRNegotiationMolecular CloningProtein ExpressionProtocolTeam LeadershipSOPSDS-PAGEScientific WritingScientific methodologyRheumatologyRT-PCRR&Dmolecular biologyMicrosoft OfficeDNADiagnosticsCell CultureBiotechnology
Skills and Expertise
Create SOPs Develop protocols Interpret data Molecular Diagnostics Technology researchAdministrative supportAnalyze dataAssist with experimentsAssist with proceduresAssist with routine testsAttend seminarsbudgeting of R&D activitiesCollaborate with medical teamCollaborate with principal investigatorCollaborate with project teamData miningData validationWritten presentationData verificationDesign scientific projects (in biology)Execute scientific projectslifescienceManuscript preparation and reviewNetworkOffice managementObtain grantsOperate research equipmentOral presentationOrganize scientific projects (in biology)Publication of articlesProvide trainingPublish scientific projectsReport dataSetup of research equipmentSolve problemsUse western blotting techniqueDirect co-workersAntibody CharacterizationRecombinant DNA TechnologyTroubleshootingmolecular biologyMolecular BiotechnologymicrobiologyGenetic engineering
Doctorate in Recombinant Protein Technology from VIT University in 2016
M.Sc in Microbiology from Bangalore University in 2002
B.Sc in Microbiology from Bangalore University in 2000
Diploma in Genetic Engineering from Jain Institute of Vocational Studies in 1999
Training and Certification
Diagnostic techniques in 2001 Training
Reference slides for TB-RNTCP in 2001 Training
Profiiency Course in Genetic Engineering in 1999 Certification
Expert has 2 publications (Will be avalible with full profile)