I am a recent master graduate in biochemistry & biotechnology and I am looking for a practical starting function with responsability preferably in the biopharmaceutical- and/or agroscience industry. I have a strong interest and working knowledge in applied & fundamental research concerning plants, microorganisms & pharmaceuticals. I am a hands-on person with a strong motivation and commitment to improve and broaden my expertise with conventional and new molecular biological-genetical methods, techniques and machinery. I would like to contribute in a technical manner to innovative research in the above mentioned fields
Associate Scientist Cellular Pharmacology Mechelen, Belgium
November 2015 --- October 2017
Profiling of small-molecules in early drug discovery up to preclinical candidate delivery via biochemical and cellular assays.
- Running routine and advanced biochemical/cellular assays using a plethora of technologies (luminescence, fluorescence, radioactivity, high-content microscopy,...)
- Full assay data analysis and quality control
- Reporting results to project stakeholders and providing input to senior research staff
- Assay development and validation
- New technology exploration on small-molecule therapeutics
- Mammalian (primary) cell culture
- Understanding complex biological processes
Student scientific researcher Leuven, België
October 2013 --- July 2014
Master thesis research project : Design and implementation of a in vivo protein - protein interaction sensor in the opportunistic fungus Candida albicans.
Aim : Produce recombinant Candida albicans strains expressing fragments of a genetically splitted green fluorescent protein (GFP) fused C-terminally to target genes. Test whether the target proteins interact with one another by fluorescence microscopy. If target proteins interact, the fused GFP fragments recombine and reconstitute a native-like GFP. The target proteins can then be localized to a cellular compartment, in which the interaction takes place, by fluorescence microscopy.
Student scientific Researcher KU Leuven
February 2013 --- April 2013
Master research internship in the laboratory for neuronal networks and regeneration (KU Leuven). Confocal microscopic analysis of retinal ganglion cell degeneration provoked by N-methyl-D-aspartate injection of mouse retinas - a glaucoma model.
Duties: Dissection of wholemount mouse retinas - immunohistochemical staining of Brn3a (a retinal ganglion cell-specific marker) - visualization of the Brn3a marker by confocal laser scanning fluorescence microscopy - Producing high quality images of retinal ganglion cell population in retinas via Brn3a - Digital counting of retinal ganglion cells via Axiovision.
Attention to detailCritical thinkingInterest in knowledgeSociabilityIndependenceCreative thinking
molecular biologyMolecular & Cellular BiologyMolecular CloningMolecular geneticsPlant Physiologyplant biologySignal Transductionrecombinant DNA technology
Skills and Expertise
pcrCLC workbenchmolecular biologytransfectiontransformationFluorescence microscopycloningCell cultureRT-PCRSDS-PAGEWestern blottingDNA sequencing analysisConduct literature searchesMS office Analyze data
Master in Biochemistry and biotechnology from KU Leuven in 2015
Bachelor in biochemistry and biotechnology from KU Leuven in 2014
Expert has 1 publications (Will be avalible with full profile)